Expression And Significane Of Twist2and E-cadherin In Cervical Squamous Cell Carcinoma

Objective:Research the transcription factor Twist2and epithelial cells cadherin E-cadherin expression in normal cervical tissue、 cervical intraepithelial neoplasias and cervical squamous cell carcinoma, discuss them in the process of change in cervical cancer role and significance.Methods:Using immunohistochemical method (PV-9000two-step)detected Twist2E-cadherin expression in the Normal cervix group (30cases), low-grade cervical lesions (CIN I)(35cases), high-grade cervical lesions (CIN Ⅱ-Ⅲ)(44cases), cervical squamous cell carcinoma (53cases)four groups. Another60cases of cervical tissue (20cases of cervical squamous cell carcinoma,20cases of cervical intraepithelial neoplasias (CIN) and20cases of normal cervical tissue). Using Western blot detect Twist2, E-cadherin in normal cervical tissue, cervical intraeithelial neoplasias (CIN), cervical squamous cell carcinoma of the expression.Results:①Immunohistochemical method show that:Twist2and E-cadherin in normal cervical, CIN (Ⅰ), CIN (Ⅱ-Ⅲ), the positive expression rate in cervical squamous carcinoma respectively:6.7%,25.7%,47.7%,79.2%, and93.3%,74.3%,52.3%,24.5%. Comparison between groups, the differences were statistically significant (P<0.05). The positive expression rate of Twist2、 E-cadherin in cervical squamous cell differentiation, clinical stage, lymph node metastasis between the groups, the difference was statistically significant (P<0.05), no statistically significant difference between the different age groups (P>0.05)②The Western blot method showed that:Normal cervix, CIN group, cervical squamous cell carcinoma groups Twist2gradually increased E-cadherin decreased, the relative expression of differences among the three groups was statistically significant (P<0.05).③Twist2and E-cadherin expression was negatively correlated in cervical squamous cell carcinoma (r=-0.401, P=0.003)。Conclusion:Twist2, E-cadherin may participate in the process of malignant transformation of cervical squamous cell carcinoma, joint detection Twist2, E-cadherin help to determine the degree of malignacy of cerical cancer.