Effect Of Ghrelin On PepT1of The Small Intestinal Epithelial Of Rats With Sepsis

Objective To investigate the effect of Ghrelin on PepT1of the small intestinal epithelial in sepsis rats.Methods Eighty male SD rats were divided into four groups:normal group, sham operation, sepsis group and Ghrelin group, and there twenty rats in every group, by random number table.The model of sepsis was made with cecal ligation and puncture (CLP).After the procedure Ghrelin was injected via vein in the Ghrelin group. Ten rats by random in every group were used to investigate the intestinal histological. Serum and intestinal mucosas TNF-α, IL-1β and Ghrelin were detected with Elisa. In addition, Immunohistochemical, Realtime PCR and Western-blot were used to detect PepT1distribution, PepT1mRNA expression and PepT1protein expression levels respectively in every group. And the uptake of PepT1of small intestinal epithelial cells was measured with high performance liquid chromatography. The others were recorded the time of the survival of the rats in the treatment period (7d)Results (1) The levels of serum and intestinal mucosas TNF-α and IL-1β in the sepsis group were significantly higher than in normal and sham operation groups (P<0.05). The PepT1distribution, PepT1mRNA expression levels, PepT1protein expression levels and the uptake of PepT1in the sepsis group were significantly lower than in normal and sham operation groups (P<0.05).And the damage to the small intestine mucosa was more serious in sepsis group compared to normal and sham operation groups.(2)Comparing with the sepsis group, thhe levels of serum and intestinal mucosas TNF-α and IL-1β in the Ghrelin group were significantly decreased (P<0.05). The survival rate of rats, PepT1distribution, PepT1mRNA expression levels, PepT1protein expression levels and the uptake of PepT1in the Ghrelin group were significantly improved than in sepsis group (P<0.05), and a lighter damage to the small intestine mucosa.(3) There were no differences in various indicators between sham operation and normal groups (P>0.05)Conclusion The PepT1mRNA and protein expression levels with sepsis were significantly decreased, which could effect the uptake of PepT1.Ghrelin could decline the inflammatory response and greatly upregulate the physiological function of PepT1of small intestinal epithelial cells.