Alcohol Drinking Patterns And Its Relationship With The Polymorphism Of ADH2,CYP4502E1in Physical Examination Population Of Tibetan Males In Qinghai Province

Objective To investigate the factors influencing drinking behavior patterns and genetic polymorphism distribution attributes of ADH2and CYP4502E1in Tibetan males in Qinghai province in order to understand relationship between alcohol drinking pattern and these two gene polymorphism.Methods1325Tibetan males in physical examination population in Centers for Disease Control and Prevention in Qinghai Province from April to July in2010were randomly selected to complete a questionnaire survey by using self-made questionnaire which contents included human basic characteristics and drinking behaviors. At the same time, blood samples were collected to determine the distribution of the genetic polymorphism of ADH2and CYP4502E1by PCR-RFLP technique. The age of respondents was from18to85, and the average age of them was39.62±10.92.2The relationship between the drinking behavior and its related risk factors were analyzed through the statistics method of multi-way logistic regression. Furthermore, the relationship between the amount of alcohol and the distribution of the genetic polymorphism of ADH2and CYP4502E1was also analyzed by chi-square test.Results1The drinking rate in physical examination population of Tibetan males was59.38%. Single variable analysis showed that it was related to occupation, educational status, income, marital status and smoking (p<0.05). Income and smoking were independent factors.2The most common drinking time was at night in conjunction with food consumption. There were statistically significant differences between the risk drinking group and safe drinking group in the related indicators including drinking occasion, the type of alcohol, the amount of the alcohol type consumed and redness of face (p<0.05).3As for ADH2gene, there were statistically significant differences of the alleles of ADH2*1and ADH2*2between drinkers and non-drinkers (p<0.05), and for CYP4502E1gene, there were statistically significant differences between drinkers and non-drinkers in the genotypes of c1/c1, c1/c2, c2/c2and the alleles of c1, c2(p <0.05).Compared with safe drinkers, they all had statistically significant differences of the genotypes and the alleles of ADH2and CYP4502E1in the risk drinking groups (p<0.05) Conclusion1The drinking rate in physical examination population of Tibetan males is59.38%.2Occupation, educational status, income, marital status and smoking are the influencing factors on drinking behavior. Income and smoking are independent risk factors.3The drinking pattern of Tibetan males is severely affected by social contact. When drinking, most of them would not demonstrate a sign of face redness. They like to choose low-alcohol consumption at night, and accompany by food and vegetables, and they also like only one kind of alcohol.4ADH2and CYP4502E1are associated with drinking behavior in Tibetan males. The ADH2*2allele can protect drinking, but the c2allele can enhance drinking.