Experimental Study Of Lentiviral Vector Of SOX9Gene Transducing Bone Mesenehymal Stem Cells

Objucive To construct one lentiviral vector containing mouse SRY-related high mobilitygroup-box gene9(Sox9) and to transfect murine bone mesenehymal stem cells(mBMSCs) invitro and observe the expression of target gene.Methods RNA from the vectors containing mouxe Sox9gene were extracted and Sox9genes were amplified by reverse transcription-Polymerase Chain Reaction (RT-PCR).The Sox9genes were connected into lentiviral vectors pGC-FU.Then pGC-FU-Sox9transduced into293Tcells to produce recombinant lentivirus called as Lenti-SOX9-EGFP. mBMSCs weretransfected.The expression of target gene was detected by immunofluorescence,RT-PCR andWestern blot.Results Lenti-SOX9-EGFP was recombined successfully and transduced efficiently intomBMSCs.The expression of Sox9gene was confirmed by RT-PCR and Western blot.Conclusion Lentiviral vector of mouse SOX9gene can transfected successfully intomBMSCs。Meanwhile，Sox9gene may be expressed in Sox9Transduced mBMSCs.This willprovide target cells for the following study about Sox9gene respairing cartilage injury.