The Research On Extraction Of Dicranopteris Dichotoma Antimicrobial Active Ingredient And Antibacterial Mechanism

In recent years, the plants continue to be found with the antibacterial substances, based on the development of antibacterial constituents from plants into natural preservatives and natural antibacterial drugs has received wide attention, but gets much material belonging to precious herbs or essential oils. Due to the high cost of precious herbs, greatly limiting its development.Dicranopteris dichotoma has rich resources and access to cheap and so on.Dicranopteris dichotoma as the research object in this article, the use of cthanol extraction of antibacterial substances, and orthogonal optimization the extraction process, optimize the resulting extraction is the extraction temperature of40"C, time2h, the volume fraction of ethanol extract40%, solid-liquid ratio1:35. Extraction of dicranopteris dichotoma in this technology on the inhibition of Staphylococcus aureus in diameter11.50mm.Dicranopteris dichotoma antimicrobial active ingredient in size and influence factors of temperature extraction time> solid-liquid ratio> volume fraction of ethanol, extraction temperature on antibiotic extraction of effective constituents most affected.Based on optimum extraction process of extraction, silica-gel column separation order to have antimicrobial active ingredients separation and purification of filtered, by inhibition of target substance by gel column separation of component6, identified by IIPLC with a purity of more than99%.The bacteriostatic test in its concentration of0.5mg/ml, the bacteriostatie circle diameter is13.30mm.Than before the separation of ethyl acetate on bacteriostasis of1.0mg/ml circle0.1mm, under the same concentration higher than the commonly used preservatives potassium sorbate inhibitory activity.Then identification of chemical reactions. NMR and IR spectra to study the structure and type of target substances found target material may belong to the condensation tannin compounds, with a glycosidic bond. With a target substance molecular weight of about800-1000.Through the determination of growth curve of Staphylococcus aureus, SDS-gel electrophoresis for determination of protein damage, AnnexinV-FITC/PI double marking determination of apoptosis rate, scanning electron microscopic observation of cell structures and other means had a preliminary discussion on the mechanism of inhibition of target substances, Be bacteriostatic antimicrobial main function mode of the target substance is by acting on cell protein causes the cell membrane of the cells on the festering, collapse of the cytoskeleton to produce blocky, eventually led to the breakdown of the cell, in order to inhibit the growth of bacteria.The objective of this study on the dicranopteris dichotoma by Staphylococcus aureus isolation and preliminary study on bacteriostatic mechanism of components, provides a theoretical basis,for the further development of dicranopteris dichotoma.