| [Objective]Diffuse large B-cell lymphoma (DLBCL) is the most common type of non-hodgkin’s lymphoma, and to study its molecular genetics characteristics, to understand the pathogenesis and looking for better treatment methods become the hot spot of the research. To investigate the aberration of MYC gene and analyze the correlation of gene aberrations between MYC, BCL-2and BCL-6in diffuse large B-cell lymphoma (DLBCL) cases from Chinese patients, then analyze the correlation of gene aberrations of MYC and the expression of Ki67.[Methods]There are194DLBCL cases from QiluHospital and TianjinTumorHospital of eastern China were collected, then made into tissue microarrays. We give the tissue microarrays for H E staining and observed the tissue morphological characteristics of DLBCL cases under microscope, and record the morphological features of typical cases. The aberrations of MYC, BCL-2and BCL-6genes were detected using interphase fluorescence in situ hybridization (FISH) and the protein markers (CD10, BCL-6, MUM1, BCL-2and Ki67) were stained using immunohistochemistry (IHC) in tissue microarrays of194DLBCL cases. The correlations of them were analyzed using statistics.[Results]1. General characteristics:In the194cases which have complete data, there are106males and88females, from16to86years old, and the average age is58year-old. The positive rates of CD10, BCL-6, MUM1and BCL-2were17.53%(34/194),48.45% (94/194),68.04%(132/194) and62.37%(121/194). According to Hans classification method, non-GCB subtype (144cases,74.23%) was significantly more common than GCB subtype (50cases,25.77%)(P<0.001).2. Among the194collected cases,164of them have FISH results of MYC gene in the end. There are38cases (23.17%) of MYC gene aberration were detected. Among them9cases (5.49%) were MYC translocation, which7cases in GCB subtype (7/49,14.29%) that significantly more than those in non-GCB subtype (2/115,1.74%)(P=0.009). Other29cases (17.68%) were MYC gene amplification, there were no significant difference on the distribution of them between GCB (10.20%) and non-GCB (20.87%) subtypes (P=0.187).3. of the159cases with complete data of FISH tests, only two "double hit" cases with MYC and BCL-6gene rearrangement coexisted were found, no case with MYC and BCL-2genes or three of them rearrangement coexisted. There was a significant positive correlation between MYC gene amplification (28/159,17.61%) and BCL-2gene amplification (38/159,23.90%)(r=0.2916, P=0.0004).4. High expression rate of Ki67(>90%) was significantly more common in the cases with MYCC-translocation (5/8,62.50%) than those without MYC-translocation (33/149,22.15%)(P=0.0277). Two "double hit" cases were both Ki67high-expression. There were no significant correlation between MYC gene amplification and high expression of Ki67.5.We were followed up to27patients, the3year survival rate is only37.51%. Those12cases were died, non-type GCB (72.22%) significantly greater than the GCB(22.22%)(P=0.0137).[Conclusions]1. MYC gene translocation detection rate is low, only5.49%, and most occur in GCB translocation cases; MYC gene amplification oftenin non-GCB.2. There was only two "double hit" cases with MYC and BCL-6gene rearrangement coexisted were found, no case with MYC and BCL-2genes or three of them rearrangement coexisted.It needs to be added in more cases to verify. 3.MYC gene translocation of Ki67high expression rate was significantly higher than that of the translocation of cases, double-hit as high proliferation activity; MYC gene amplification and the amplification of cases there was no significant difference between high Ki67expression, its biological significance to be further research.4. This follow-up cases, The three yesrs survival rate was only slightly lower, but the prognosis of GCB subtype is better than non-GCB subtype, its relationship with MYC gene abnormalities should be followed for more patients to analysis. |
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