The Quality Evaluation Of Pseudostellaria Heterophylla Germplasm And Relevant Pharmacodynamic Study

This study in order to comprehensive and effective evaluation of the quality of pseudostellaria heterophylla germplasm, we have investigated ZheRongXian plant resource of pseudostellaria heterophylla sampling, measured its biomass index, and analyzed the main chemical components of pseudostellaria heterophylla sample collections in content established the fingerprint of n-butanol part, validated the replenishing qi to invigorate the spleen and enhance immunity effect of pseudostellaria heterophylla experiment. Used SPSS18.0software quality of pseudostellaria heterophylla biomass index and analysis indicators of comprehensive analysis to evaluate the quality pseudostellaria heterophylla germplasm.1、For ZheRongXian cultivated pseudostellaria heterophylla resource investigation, and in the form of questionnaire have understood way of pseudostellaria heterophylla grown, fertilizer applying pesticide and plant diseases and insect pests, etc., by random sampling method to collect a large number of sample data, and the biomass of pseudostellaria heterophylla were determined and analyzed index. Results showed that the main problem is in the planting of pseudostellaria heterophylla continuous cropping obstacle, purple stripes feather disease and leaf spot, etc.;Jiangsu and guizhou in ZheRongXian plant yield is relatively low; In a different group of pseudostellaria heterophylla grown in production has been a significant difference; pseudostellaria heterophylla production with root tuber numberr and fresh weight of aerial part of a piece have a close relationship.2、Established pseudostellaria heterophylla more index quality analysis system, determinaed contents of polysaccharide and total saponin in pseudostellaria heterophylla by the ultraviolet spectrophotometric method, determinaed contents of cyclic peptide A(HA), and cyclic peptide B (HB) pseudostellaria heterophylla by HPLC method. Measured the content of total polysaccharide is higher, the total saponin content is relatively stable, HA and HB content in pseudostellaria heterophylla differences, provenance differences between different regions is most obvious, is consistent with the reports in different regions of the differences. Application of HPLC method was developed to establish the fingerprint of pseudostellaria heterophylla n-butanol extraction, analysed30batches of different origin pseudostellaria heterophylla HPLC fingerprint, the similarity degree is not high, calibration13common peak, analysed the peak area of the principal component, and cluster analysed by principal component score value, The germplasm resources of pseudostellaria heterophylla from different habitats were different in the n-butanol chemical.3、To study the replenishing qi to invigorate the spleen and enhance immunity effect of pseudostellaria heterophylla. Established the "spleen deficiency" mice model replicate by rhubarb with their diarrhea remedy, and weighted loading swimming time, cold temperature changes, and hypoxia time index in mice to reflect the anti-stress ability; determinated mice devouring index and coefficient, and analysed pseudostellaria heterophylla effects on immune function. Two groups of experiment results show that the four different origin pseudostellaria heterophylla aqueous extract of rhubarb to spleen deficient model can be used to improve spleen deficient of mice, improve effect has a tiny difference between different regions, but no significant difference.4、By SPSS18.0software, analysed the biomass index, quality index and pharmacodynamics index by correlation analysis, principal component analysis and cluster analysis, comprehensive analysis and evaluate the quality of pseudostellaria heterophylla germplasm. biomass index and chemical index of the polysaccharide content is significant correlation. Clustering analysis quality ZheRongXian pseudostellaria heterophylla germplasm can be divided into four categories, and sreened out five better samples.