Experimental Study Of Diaphragmatic Stasis Expelling Decoction Reverses The MDR-1Expression Of Nude Mouse Transplantation Tumor And Clinical Research Of Reversing The MDR-1Expression Of The Recurrence And Metastasis Of Colorectal Cancer

Objective:1. Experimental study Of Diaphragmatic Stasis Expelling Decoction reverses the MDR-1gene expression of nude mouse transplantation tumor;2.Clinical Research of Diaphragmatic Stasis Expelling Decoction reverses the MDR-1gene expression of the recurrence and metastasis of colorectal cancer.Methods:1. Determined by MTT method to detect HCT-8, HCT-8/5-FU tumor cells of four kind of mechanisms of action different chemotherapy drug resistance (5-FU, VCR, VP-16, DDP), The logarithmic phase HCT-8/5-FU right armpit, cell inoculation in nude mice treated tumors had an average volume to100mm3,28nude mice were randomly divided into four groups: control group, Diaphragmatic Stasis Expelling Decoction group, the5-FU group, Diaphragmatic Stasis Expelling Decoction group+5-FU group. Every two days to measure the longest diameter of the tumor and the shortest path, calculation of tumor volume; Mice were euthanized at continuous dosing after14days, or in tumor tissue, weighing. Rt-pcr respectively detect MDR1mRNA expression in tumor tissue.2.Late into the group of50cases of recurrence and metastasis of colorectal cancer patients, randomly divided into2groups, one group with FOLFOX4program4chemotherapy cycles, a set of FOLFOX4program under the diaphragm by stasis soup add4cycles of chemotherapy. Before chemotherapy and after chemotherapy respectively to collect peripheral blood2ml. RT-PCR method, the detection of peripheral blood of MDR1gene expression. The results in the SPSS16.0software for statistical analysis.Results:1. MTT methods was to detect drug-resisitant of human colonic cancer cells HCT-8/5-FU to5-FU、DDP、VCR、VP-16are120.94+6.93、11.47+0.13、11.74+0.2、21.11+0.60. The difference was statistically significant (P<0.05). Show that resistant cells to other different mechanism, structure to some extent effective cross resistance. The drug resistance of tumor cells detection, transplantation, HCT-8IC50forO.26+0.12, HCT-8/5-FU IC50for26.56+0.23. The results show that of the in vitro drug resistance ratio declined slightly, but still maintain good resistant properties. 2.HCT-8colonic carcinoma transplanted tumor into tumor at a rate of71.42%(5/7), HCT-8/5-FU cell into a tumor drug resistance rate was88.23%(30/34), they has no obvious difference.3. Tumors growth rate of Diaphragmatic Stasis Expelling Decoction group and Diaphragmatic Stasis Expelling Decoction add to5-FU group are significantly slower than blank control group and5-FU group. Compared with the blank control group and5-FU group difference was statistically significant (P<0.05).4. Tumor inhibition rate of Diaphragmatic Stasis Expelling Decoction group and Diaphragmatic Stasis Expelling Decoction add to5-FU group are9.6%andl8.0%, Tumor weight block respectively (3.17±0.53)g and(2.88±0.31)g, Were significantly lower than the control group respectively (P<0.03and P<0.05), suggesting under Diaphragmatic Stasis Expelling Decoction can inhibit the growth of xenograft tumor in nude mice.5. The MDRlmRNA of Diaphragmatic Stasis Expelling Decoction group is significantly lower that control group.The expression level of Diaphragmatic Stasis Expelling Decoction add to5-FU groups MDRlmRNA is lower that control group, with statistical significance (P <0.05).6. Pure chemotherapy group of peripheral blood of MDR1expression is76%before chemotherapy, chemotherapy MDR1expression is84%, and increased before treatment after chemotherapy, the MDR1expression difference was statistically significant (P<0.05), suggesting that the chemotherapy of MDR1expression in patients with recurrent metastatic colorectal cancer has increased trend, prompt acquired secondary drug resistance after chemotherapy.7.Chinese medicine combined with chemotherapy before the chemotherapy group peripheral blood of MDR1expression is68%, MDR1expression is40%after chemotherapy, chemotherapy compared with before treatment is reduced, the MDR1expression difference was statistically significant (P<0.05), shows that under the diaphragm by stasis soup has reverse tumor drug resistance related to secondary drug resistance.Conclusion:1.Multi-drug resistant cells for a drug resistance, have not come into contact with, structure, mechanism of different variety of antitumor drugs also have cross resistance;2. Subcutaneous transplantation is to establish a multi-resistant nude mice transplantation tumor of the good method, with simple operation, stable resistance characteristics, as the characteristic of high rate of tumor;3.RT-PCR results showed that under the diaphragm by stasis soup reversible nude mice transplantation tumor of MDR1gene expression, but its mechanism needs further research;4.1ncreasing tumor cell drug resistance, and chemotherapy may be related to acquired secondary drug resistance after chemotherapy5.Under the Diaphragmatic Stasis Expelling Decoction group combined with chemotherapy can improve the efficiency of clinical chemotherapy.