HPV Virus Detection In Cervical Lesions Of ASCUS Diagnostic Value Is Discussed

Objective:To use hybrid capture the second generation test technology to the cervical liquidcytology (Thinprep cytology test TCT) test results for meaning clear not typical squamousepithelial hyperplasia (ASCUS) patients were done HPV-DNA virus detection. EvaluationHPV virus load and cytology results for cervical lesions ASCUS occurred in the degree ofcorrelation, this paper discusses the HPV virus load testing in ASCUS patients reasonabledivergence and the standard management of clinical value.Methods:A retrospective analysis in January2010-2011ovember period, medical center inTaiAnShi hospital and people’s hospital aternity clinic status line liquid and cytologypatients, cytology diagnosis standard classification system by TBS, for screening results forcan’t clear the meaning is not typical squamous epithelium (ASCUS) of the disease in924cases, the application of the second generation hybrid capture test technology, lineHPV-DNA viral load test, respectively by viral load1.0-10for low viral load,10-100infor viral load,>100for high viral load for grading standards, all cases simultaneouslyvaginal mirror inspection, and suspicious blockage in designated biopsy or line ECC topathology results as the gold standard in the diagnosis, and the result of tissue pathology,evaluation to HPV-DNA viral load as the basis, and study the ASCUS patients with thecervical intraepithelial neoplasia of existing correlation sample lesions by discussesapplication testing HPV-DNA viral load in ASCUS reasonable divergence in patients withmanagement application value.Results:The inspection results of TCT TBS (The Bethesda System) classification System,including detection can’t clear meaning is not typical of The squamous epithelial cells(Atypical squamous cells of undetermined significance ASCUS)924cases of7.08%. Out of the924cases of screening for patients with cytology ASCUS HPV-DNA test for this object,including HPV fiberobronchoscopically collected tissues or for240cases, happen in144cases CIN number, negative number for684cases of HPV, HPV infection rate was25.97%,not when HPV infection cervical intraepithelial neoplasia of the change rate of2.34%, whenthe CIN HPV infection rate of60%, which shows a significant difference. HPV in theASCUS sensitivity of90%, negatie predictie alue was97.66%, the positie predictie alue was60%, specificity of87.43%. In the low viral load CIN in the incidence of11.66%, moderatein the viral load CIN was22.5%, and high in the viral load CIN rate of45%, the differenceof the P <0.05.Conclusion:1. ASCUS the diagnosis covers noodles wide, repeatability is poor, on biopsy tissuepathology diagnosis with diversity, can contain the height of the underlying disease,effective screening out one of the cervical lesions and the standardization management toreduce the incidence of cervical cancer has important significance.2. HPV infection in patients with positive-DNA, the odds of cervical CIN, high viralload cervical high level cervical lesions occur likely to increase.3.In the ASCUS are fuzzy cytology diagnosis, can undertake HPV hb iral load test,may to the existence of the patients with cervical lesions shunt management, can promptdiagnosis and treatment, avoid the diagnosis of patients at high risk.4.The rationalization of cervical lesions on screening, can effectively reduce the furtherdevelopment of the lesion before cervical cancer.