Based On LC-MS And Metabolic Profiling In The Evaluation Of Atractylodes

Atractylodes is the dry rhizome of Atractylodes macrocephala koidz.The beginning carries in "the house of god BenCaoJing".Atractylodes has the glorious use history in our country.Nature painstakingly,sweet,Atractylodes is good for the spleen, it can stop the perspiration, and the embryo.。At present in most parts of the country in cultivation largehead atractylodes rhizome。Atractylodes macrocephala koidz which is Planted In zhejiang yuti and anhui wannan is the authentic medicinal herbs.In ancient times,genuine traditional chinese drugs has been regarded as a high quality medicinal herbs.at that time,the people had known that the environment may affect the quality of the Raw material for medicine.As a result of condition limit,the people is unable to research and study this problem.but now a lot of reach of the Atractylodes Concentrates in The cultivation and management of Atractylodes macrocephala, The formation of the authentic medicinal herbs of Atractylodes,the studies on the chemical constituents of Rhizoma Atractylodis Macrocephalae, The pharmacological effect of Atractylodes macrocephala and so on.and we do not pay more attention to the reach of Distinction of Bai Zhu to cultivate and the typical raw material for medicine.Along with LC-TOF-MS rapidly expand,It has become a Powerful method for the reach of protein,the analysis of Natural extraction and Medicine generation of dynamics and so on.We use the liquid chromatography/mass spectrum analyzes the Bai Zhu methyl alcohol extraction, and use the stoichiometry method (Cluster analysis and Principal components analysis)to analysis four sample’s spectrograms. According to the molecular weight information of organic component to judge the difference of kinds of organic components between large-headed atractylodes medicinal materials.This paper applied LC-TOF-MS technology analysis and determine four kinds of atractylodes, one is gathered from QiMen, and the others are purchase from bozhou. we devotes in discussing the distinction of Bai Zhu to cultivate and the typical raw material for medicine. Through the LC-TOF-MS analysis,detect nearly100most of the components in the above four atractylodes macrocephala koidz, their content is close, but have the individual differences in composition. use the principal component analysis to analysis four spectrograms, we can Discriminate four kind of atractylodes and find the distinction between chemical composition.In addition, the clustering analysis of spectrogram shows the same result. Based on four samples by principal component analysis, that is what caused the difference between these four samples, by spectral analysis and chemical composition of compare and find, cause wild Atractylodes and cultivated Atractylodes difference component may be of alkaloid and polysaccharide composition. Which may wild Rhizoma Atractylodis Macrocephalae and imitation of wild contains alkaloids, and cultural goods without. Wild Atractylodes and cultivated all contain the polysaccharide component, difference is that wild Atractylodes containing polysaccharide component ratio of planting products.In addition, through the analysis and comparison of if imitation wild Atractylodes fractions and wild differ not quite, can be cultivated Atractylodes rhizome shifting to the wild, increase of Atractylodes macrocephala in medicine, clinical application.Wild Atractylodes macrocephala with cultivation between whether other metabolites are different, requiring subsequent experiments demonstrated...