Reserch On Myoblast Cytocompatibiiity With PLGA Biomaterial After Transfection Of CDMP-2Gene

Objectiveto study the biocompatibility of PLGA with Myoblast which transfected with CDMP-2Gene.MethodsSkeletal muscle from limbs of5days neonatal SD rats was obtained, from which neonatal rat myoblasts were isolated and harvested by direct mixed enzyme digestion. Then the cells were further purified by combination of differential velocity adherent and trypsin ization methods. The morphologic property and growth state of the myoblasts were observed. Myoblast transfected with CDMP-2Gene cells were inoculated on PLGA stents with four groups:A.Myoblasts;B.Myoblast transfected with CDMP-2Gene cells;C.Myoblasts and PLGA stents; D.Myoblast transfected with CDMP-2Gene cells and PLGA stents. The bioactivity of myoblasts on the PLGA was examined with the method of MTT, myoblasts growth curves were drawn, and desmin expression was detected.ResultsWhen cells were inoculated on PLGA stents in the early period, only fiber network structure was observed, and cells growth and adhesion on the surface of stents could not be seen in the vision of inverted contrast microscope. The cells proliferated well after72hours and had a significant increase in quantities. With the method of MTT before and after transfection, it showed that the4 groups of light wavelength absorption values at492nm respectively were0.046414,0.057665,0.038945,0.04991; while at the wavelengths629nm were0.132158,0.178811,0.108284,0.173810. A.group B.group C.Group andD.Group, there were usually no significant difference statistically (p>0.05).ConclusionsThe myoblasts proliferate well on PLGA stents, which indicates that nontoxicity of PLGA materials for myoblasts, and excellent biocompatibility of PLGA can be used for tissue repair. It is hoped that an analysis of this experiment will provide a new prospective for a better understanding on the repair of cartilage damage.