Study On Growth Of Laryngeal Cancer Cell Hep-2by Aidi Injection

ObjactiveTo study the effect of Aidi，A chinese medicine, on growth and apoptosis oflaryngeal cancer cell line Hep-2.MethodsHep-2cells were treated with Aidi at0.4,0.8,1.0,2.0ug/ml concentrationsas experimental groups,0ug/ml as control group.After24、48and72hours,MTTassay was evaluated in the cellular proliferation inhibition rate, fluorescencemicroscope was testing in the apoptosis,the cell cycle distribution and thechanges of apoptosis ratio were analyzed by flow cytometry,and real-timefluorescent quantitative PCR was used to detected the mRNA expression ofCyclooxygenase-2in the Hep-2cells.Resultsâ‘ Aidi at different concentration inhibited the growth of Hep-2cells in asignificant time-and dose-dependent manner compared with that incontrols（P<0.05）. The growth inhibition rate at0.4、0.8、1.0and2.0μg/mL for72h were23.08%、37.42%、43.38%、54.48%.â‘¡The typical apoptosischaracter-istics was found in the experimental groups from Fluorescence,and normal cellswas significantly reduced. The control group didn′t observe apoptosis changeand the chromatin in cells evenly distributed.â‘¢FCM showed that Aidi decreased the apoptotic rate of Hep-2cells at the G₀/G₁phase, increased the apoptoticrate of Hep-2cells at the G₂/M phases and induced the apoptosis of Hep-2cells.The apoptotic rates at0.4、0.8、1.0and2.0μg/mL for72h wereincreased28.62±1.23%increased54.90±2.40%, respectively, which were allsignificantly higher than those of the control group（P<0.01）.â‘£The expressionlevel of epoxidase was higher in Hep-2cells than in control72h after treatmentwith Aid. the mRNA expression of Cyclooxygenase-2in the Hep-2cells weredecrease by real-time fluorescent quantitative PCR （P<0.05）.ConclusionsAidi inhibits the growth of human Hep-2cells by inducing apoptosis of them.The mechanism is related of the mRNA expression of Cyclooxygenase-2through it is cut...