Effects Of IGF-IR Monoclonal Antibody On The Proliferation And Apoptosis Of Jurkat Cell Line

Background Acute lymphocytic leukemia(ALL) is the most common malignant tumor in childhood. The traditional chemotherapy lacks of tumor specificity and the mortality rate related to chemotherapy is high. The insulin-like growth factor-I receptor(IGF-IR) are overexpressed in a variety of malignant tumor cells and participate in the occurrence and development of cancer.It can speculate that the childhood ALL cell line Jurkat may also overexpress IGF-IR and the monoclonal antibody(MAb) targeting IGF-IR could inhibit the growth of Jurkat cells. In this topic we will make some relative research.Objective To investigate the expression and cellular location of IGF-IR in Jurkat cell line.To explore the effects of IGF-IR MAb on the proliferation and apoptosis of Jurkat cell line.MethodJurkat cells was cultured in RPMI1640which contains10%fetal bovine serum(FBS).1. The expression of IGF-IR in Jurkat cells was detected by immunocytochemistry.2. Jurkat cells were incubated with various concentrations of IGF-IR MAb of0.001μg/ml,0.01μg/ml,0.1μg/ml,1μg/ml,10μg/ml for48hours. The effect of IGF-IR MAb on the proliferation of Jurkat cells was tested by CCK-8assay.3. According to the result of CCK-8,we chose the concentration of10μg/ml of IGF-IR MAb to treat with Jurkat cells.After48hours,the apoptosis rates of Jurkat cells were detected by flow cytometry(FCM).Result1. IGF-IR was generally expressed in Jurkat cell line and the positive expression rate of IGF-IR was100%.The IGF-IR was mainly expressed as a mixture of both cell membrane and cytoplasm staining. 2. The proliferation of Jurkat cells was inhibited after treated with different concentrations of IGF-IR MAb of0.001μg/ml,0.01μg/ml,0.1μg/ml, lug/ml,10μg/ml for48hours, and the inhibition rates were respectively (9.67±1.17)%,(14.89±1.06)%,(17.64±0.81)%,(20.15±1.14)%,(24.10±1.11)%. Along with the increasing concentration of the IGF-IR MAb, the proliferation inhibition rate of Jurkat cells also increased. Significant statistics difference was found in multiple comparison (P <0.05).3. After treated with IGF-IR MAb at the concentration of10μg/ml for48hours, the early apoptosis rate of Jurkat cells was (13.73±1.16)%, the late apoptosis rate of Jurkat cells was(20.44±2.47)%,the total apoptosis rate of Jurkat cells was (34.l8±3.41)%.In control group, the early apoptosis rate of Jurkat cells was (6.27±0.67)%, the late apoptosis rate of Jurkat cells was(10.18±0.81)%,the total apoptosis rate of Jurkat cells was (16.45±1.38)%. The early apoptosis rate, the late apoptosis rate and the total apoptosis rate were all higher than that in control group. Compared with the control group,the differences had statistical significance(P<0.05).Conclusion1. The IGF-IR was generally expressed in Jurkat cell line and the IGF-IR was mainly expressed as a mixture of both cell membrane and cytoplasm staining.2. IGF-IR MAb could inhibit the proliferation of Jurkat cells in a concentration-dependent manner.3. IGF-IR MAb could promote the apoptosis of Jurkat cells.