To Study The Relationship Between Proliferation Of The Schwann Cells And Expression Of Transforming Growth Factor Beta In Facial Nerve Crushed Injury

ObjectiveTo observe the changes of transforming growth factor beta (TGF-β) expression and the rule of the schwann cells proliferation after rabbit facial nerve crushed injury and to learn about the mechanism and the correlate of TGF-βand Schwann cells in facial nerve injury and repairMethodsTwenty-four rabbits were used. The facial nerves were curshed with a special clamp. The histology and immunohistochemistry examination were performed respectively after1d,3d,7d,14d,21d,28d injury.ResultsOn3th day after injury, the TGF-β antibody in crushed facial nerve was positively expressed.and the Schwann cells increased, with the number of105.0±9.8; On7th day after injury, the Schwann cells increased greatly and the TGF-Pexpression enhanced,with the number of158.0±12.1. On14th day after injury, the expression of TGF-β decreased gradually and the Schwann cells no increasing, with the number of136.0±9.1. On21th day after injury, the Schwann cells and the TGF-Pexpression was decreased greatly, with the number of109.0±5.2; On28th day after injury, The Schwann cells and the TGF-Pexpression continued to decreased, near to normal.with the number of85.0±9.9.ConclusionsThe TGF-β participates in the pathologic process of facial nerve damage, and correlate to the proliferation of Schwann cells.