Electrochemical Detection Of Propranolol Hydrochloride On Fferent Chemicalltwo Diy Modified Electrodes

Propranolol hydrochloride (commonly known as propranolol, PRO) is aβ-adrenergic receptor blocker class of drugs, the chemical name is1-isopropyl-amino-3-(1-naphthyloxy)-2-propanol hydrochloride. This drug with sedation,frequently in clinical practice for cardiovascular disease, bronchial and vascularsmooth muscle relaxant effect and has a good therapeutic effect on diseases such ashypertension, angina, arrhythmias, and myocardial infarction. It is understood, suchas shooting and archery of the lower activity but the skill and accuracy in demandingsports events, some athletes to improve race performance on the choice of takingthese drugs to stabilize the nerve, reducing heart over-excited, reduce anxiety and soon. Therefore, propranolol hydrochloride as a β-blocker stimulants, is listed asathletes of prohibited substances in some sports items by the international anti-dopingorganizations. Therefore, the analytical methods established efficiently andsensitively for detecting propranolol hydrochloride is very important.In this thesis, propranolol hydrochloride is studied by two kinds of chemicallymodified electrodes for detecting its electrochemical behavior and its quantitativeanalysis, electrochemical analysis methods is more sensitive, simple, accurate andapplied to determine actual samples. Chemically modified electrodes combined withthe characteristics of the platinum nanoparticles, carbon nanotubes, calixarenesignificantly improve the electrochemical activity of propranolol hydrochloride.The first chemically modified electrode is used by platinum nanoparticles andmulti-wall carbon nanotubes mixture to modify glassy carbon electrode (PtNPs/MWNTs/GCE), PRO is measured electrochemical behavior by cyclic voltammetry(CV). The result indicates that in pH7.0phosphate buffer solution, PRO can exhibitan irreversible reduction peak at potential1.20V(vs. SCE). Under the optimizedconditions, PRO of quantitative analysis is measured by differential pulsevoltammetry (DPV)，the result shows that the peak current (I) and the concentrationover the range of0.2~15mg/L is a good linear relationship, and the detection limit is0.03mg/L. The methods can be used for determining the drug in the actual tablets,the sample of recovery rate is95.8~108.2%, RSD is2.51~3.05%. The second chemically modified electrode is used by p-tert-butylcalix8areneand multi-walled carbon nanotubes mixture to modify glassy carbon electrode(pTBC8/MWNTs/GCE). The result indicates that in pH7.0phosphate buffer solution,PRO can exhibit an irreversible reduction peak at potential1.18V(vs. SCE) by cyclicvoltammetry(CV). Under the optimized conditions, PRO of the peak current (I) andthe concentration over the range of1.0~16.0mg/L is a good linear relationship, andthe detection limit is0.04mg/L. The methods can be used for determining the drug inthe actual tablets, the sample of recovery rate is96.4~102.4%, RSD is2.85~3.49%.