Experimental Research Of Protease Activated Receptor-2and SC5b-9in Anaphylactic Shock Cadaver

Died of anaphylactic shock in the forensic identification in the morecommon.In recent years,relates to the medical disputes is increasing year afteryear died of anaphylactic shock.Autopsy only general signs died ofanaphylactic shock,No pathological special change.Therefore to the forensicidentification of great disorder. In the identification to diagnose anaphylacticshock death should eliminate foundation of violent death, disease,poisoning.Then combining the contact allergen history, clinical manifestation,and the content of serum IgE, mast cell tryptase, chymase, histamine, Pmaterial. Trying to died of anaphylactic shock the forensic diagnosis standardof detailed and morphological evidence identification. This experiment wasconducted based on Protease activated receptors-2and SC5b-9in theexperimental allergic shock the dead body of the experimental research.（1）Protease activated receptor is a member of the G protein-coupledreceptor family. At present, in mice and humans were found in4kinds ofProtease activated receptor. Respectively PAR-1, PAR-2, PAR-3, PAR-4. In1991,1994,1997,1998their genes have been cloned. Research shows, Almostall of the expression of PARs involved in allergic reactions in cells.Theexpression and localization of PAR-2in the body than the other three receptor.So we detected PAR-2in died of anaphylactic shock in the expression. In orderto provide basis for diagnosis of anaphylactic shock death further.EG: Forensic pathological diagnosis of anaphylactic shock,46cases of death.CG: Non-allergic shock and death in12casesMethods by immunohistochemical staining of protease activated receptor-2. Results the pathological diagnosis of anaphylactic shock death case.Renal tissue under the microscope to see a lot of protease-activated receptor positive-2staining cells.The membrane has a lot of brown granules. Simple cuboidalepithelium stained positive cells were mainly located in the renal proximaltubule and distal convoluted tubule.In other tissues did not see the positivestaining cells. Non-allergic shock and death case.No staining positive cells inlung, kidney and other tissues. There was significant difference between theexperimental group and the control group（p<0.05）.The difference wasstatistically significant.（2）The existence of SC5b-9in the plasma. For non soluble.The finalproduct is the complement system activation. So the determination of its serumconcentration can directly reflect the activation of the complement system.Thecomplement system in the human body is a complex of the innate immunesystem. Through the classical pathway and alternative pathway,mannan-binding lectin pathway is activated.To produce a series of cascadeeffect.No matter what kind of way through activation of the complementsystem. Have allergy toxin C3a and C5a in the cascade process.C3a and C5acan be combined with mast cells and eosinophils anaphylatoxin receptorbasophil cell surface expression. Stimulation of mast cells, basophilsdegranulation.The release of histamine and other vasoactive mediators. Thesymptoms of allergic reaction. Can directly stimulate vascular smooth musclecontraction.The increased vascular permeability.And on the expression ofSC5b-9in human body. The lack of further study.So we detected SC5b-9indied of anaphylactic shock in the expression.In order to provide basis fordiagnosis of anaphylactic shock death further.EG: Forensic pathological diagnosis of anaphylactic shock,46cases of death.Due to the influence of hemolysis and other factors.Can be used to detect asample of only22cases.CG: Non-allergic shock and death in12cases.Can be used to detect a sample of only8cases.With the content of Western Blot was detected in the blood of SC5b-9.Results the expression level of SC5b-9in anaphylactic shock death case in theblood samples was significantly higher than the expression level of non-allergic shock death case of SC5b-9in the blood samples. There was significantdifference between the experimental group and the control group（p<0.05）.Thedifference was statistically significant.Conclusions:（1）In the case of death from allergic shock. Protease activated receptor2in renal tissues of abnormal. Preliminary identification of protease activatedreceptor2is involved in the pathogenesis of allergic shock.（2）In the case of death from allergic shock. Blood levels of SC5b-9markedly increased.SC5b-9predecessor complement was activated.Preliminary identification of complement in some part of anaphylactic shock.