| Objective:To examine LIN28B and C-myc expression in pancreatic cancer cells.Provide new ideas for the diagnosis and treatment of pancreatic cancer.Methods:A cohort of40pancreatic tumor and10normal pancreatic samples was collected from specimens that had been surgically resected from January2008to October2011at Sichuan Academy of Medical Sciences&Sichuan Provincial People’s Hospital with the consent of patients, according to an Institutional Review Board-approved protocol. The tumor group was constituted of26male and14female, including12well differentiated samples,21moderately differentiated sample and7poorly differentiated samples. The age was arranged from36to76yeas, and the average age was62. The clinical pathological grade of pancreatic tumors was referred to UICC (2002.6th version) and was classified as18TNM I,10TNM II,7TNM and5TNM IV). As control group, the10normal pancreatic tissues were obtained from surgical operations of8lower Bile duct cancers and2duodenal cancers.The expression of LIN28B and c-Myc was detected and compared in both groups using IHC. Their expression was further analyzed to clarify possible influence on TNM grade, tumor differentiation and lymph node metastasis.Results:LIN28B and c-Myc were stained in28samples (70%) and26samples (65%), respectively, while the other cases were not stained. Both proteins were located at cytoplasm and/or nucleolus of pancreatic tumor cells. However. LIN28B and c-Myc were not seen in normal pancreatic tissues.The statistical results showed that LIN28B was inversely correlated with the differentiation status of pancreatic tumors (p<0.05), however, having no impact on TNM grade, tumor size and lymph node metastasis. Unexpectedly, our results indicated that the expression of c-Myc was not correlated with all the incoming clinical parameters. Lastly, we found that the expression of LIN28was positively correlated with c-Myc.Conclusion:LIN28B and c-Myc were stained in70%and65%tumor samples, respectively, which suggesting tumor-promoting roles of both proteins in pancreatic carcinogenesis. Higher expression of LIN28B might maintain the sternness of pancreatic cancer. LIN28B was an important downstream target of c-Myc. |
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