From The "Bowel Disease" Pathology Of Lung And Colon Tissues Of Rats And Discussion On VIP, SP, Cgrp Expression "Bowel Disease And Lung"Molecular Mechanism Es

ObjectivesThis paper discusses "bowel disease and lung" pathological change of the mechanism through the observation of bowel disease rat models of colon and lung tissue pathology by changing gastric bowel function, pulmonary function and neuropeptide VIP, SP, CGRP the expression.MethodsTake SD rats, male, a total of60. According to the weight at random into control group (24) and treatment group (36). All the rats are conventional raised a week. Treatment group use TNBS (trinitro-benzene-sulfonic acid) induced inflammatory bowel disease model; control group uses physiological saline instead of TNBS. Repeat the above method every seven days until the end of the experiment. Observe spirit, food taken, night soil and weight of the rats during the experiment. In the8th,29th,50th day after making up models put to death rats, after, measuring stomach, lung function. Then observe the change of colon, lung, heart, liver, spleen and kidney pathological morphology organization after HE staining by using microscope; colon and lung tissue ultrastructural change are observed by electron microscope, colon and lung tissue slices VIP, SP, CGRP expression are detect ed by immunohistochemical method.Rresults1pathological formLight microscope:Treatment group, colonic structure appear in the erosion of multifocal mucous membrane, submucosal and muscularis inflammatory cell performance with the pathological characteristic of ulcer after8days; colonic mucosa multifocal erosion, submucosal and muscularis inflammatory cells appear soakage after29days, colonic mucosa appeares uncharacteristic epithelial cells, the mucosa and the submucosa appears lymphocytes and plasma cells infiltration, in small vascular proliferation, visible serous layer fibre hyperplasia after50days; bronchial lung epithelial cells appear degeneration and necrosis, fall off, intraluminal inflammatory and performance in treatment group; there is no pathological morphology change in heart, liver, spleen and kidney organization in treatment group; there are no pathological changes in colon, lung, heart, liver, spleen and kidney in control group.Electron microscope:Colonic mucosa layer disappear in8th day in treatment group, only exist cellular structure outline with degeneration necrosis epithelial cells; microvilli part disappear, markedly swollen mitochondria, muscle layer intravascular mononuclear cells increase and the red blood cells appear in29th day; colon tissue mucosal appears epithelium empty bubble, degeneration and necrosis, colonic mucosa layer with only the collagen fiber organization and the exist a lot of bacteria, the mucous membrane cells become pyknotic, degeneration, inflammatory apart in50th day; treatment group lung tissue structure mitochondria swelling, with the nucleus a few empty bubble, the lung tissue appears in red blood cells in the8th day; visible in lung capillary mononuclear cells increased, epithelial cells falls off, lost, the nucleus appeared in more empty bubble in the29th day; lung mesenchymal cells composition becomes less, endothelial cells markedly swollen mitochondria, â…¡type epithelial cells microvilli loss in the50day. There is no abnormalities in control group.2pathophysiologicalLung function:compared with control group, the treatment group rats have no breathing change (P>0.05), but humidity and every minute ventilation is reduced(P <0.05) in the8th,29th,50th days; Compared with the8th day, made model, model rats, made the50day29, there is no breathing change in the29th,50th day in treatment group, while humidity have difference (P<0.05) in the29th day, humidity and each minute ventilation significant difference (P<0.01) in the50th day. Gastrointestinal function:compared with control group, the treatment group rats, exist this condition:retention rate in the stomach increases significantly and motional rate reduced of small intestine significantly in the8th,29th and50th day, which exist significant difference (P<0.01); compared with the8th day, retention rate in the stomach increases and motional rate reduced of small intestine in the29th and50th day intreatment group.3neuropeptideCompared with control group, the treatment rats, in the8th day, colonic structure, the SP VIP express rising (P<0.01or P<0.05) while in the29th,50th day, VIP, the SP express reduce (P<0.01or P<0.05). Control groups in the8th,29th and50th day, colon tissue CGRP increased with significantly difference (P<0.01); in the8th,29th,50th day, SP, CGRP expression lung tissue increased (P<0.01or P<0.05) while in the8th day VIP express no change (P>0.05),29th,50th day VIP express rising (P<0.01or P<0.05).Compared with the8th day, colonic structure, the SP expression VIP reduce (P<0.01or P<0.05), CGRP express rising (P<0.01or P<0.05) in the29th,50th day; Compared with the29th day, lung tissue VIP, SP, CGRP express rising (P<0.01or P<0.05) in the50th day (all above are in treatment group).Conclusions1bowel disease rat can appear lung function and lung tissue pathology morphology change which shows bowel disease may progress disease to the "lung"2bowel disease rat exist lung tissue pathology change after8days of molding shows bowel disease can change to the "lung" in a short time.3bowel disease rat appears colon organization, the lung tissue appears pathological form change after8,29,50days of molding while heart, liver, spleen and kidney organization stay the same shows colon and lung exist relevance.4preliminary findings VIP, SP, CGRP neuropeptide matter are part of the material basis of "bowel disease and lung", which supplies basis experiment theory for "the lung and the large intestine being interior-exteriorly related".