Role Of The Methylation Of GPX3Gene In Cervical Cancer

Objective: To Investigate the role of GPX3gene methylation in cervical cancer, wedetect the expression of GPX3in135clinical specimens and6cell lines comingfrom the cervix with real-time quantitative PCR, immunohistochemistry andwestern blot. We detect the methylation status of the the GPX3gene promoterregion in cervical cells and cervical tissue by MSP. We use5aza-dC to induce thecervical cancer cell lines demethylation, then detect their methylation status and thechanges of their biological behavior.Method:1.135samples were collected, in which63samples with normal cervical tissues and72samples with cervical cancer. The expression of GPX3was detected by real-timequantitative PCR and immunohistochemistry in mRNA and protein level.2. We detected the expression of6cell lines coming from cervix by real-timequantitative PCR and western blot.3. The genomic DNA of these4cervical cell lines and135clinical specimens was extracted and treated with bisulfite, then we detected the detect the methylationstatus of the the GPX3gene promoter region by MSP.4. We use5aza-dC to induce the cervical cancer cell lines demethylation, then detecttheir methylation status and the changes of their biological behavior.Result:1.The expression of GPX3is much more reduced in cervical cancer tissues than thenormal cervical tissues. During the process of normal cervix developing to cervicalcancer, the expression of GPX3was gradually decreased.2. The expression of GPX3was negatively correlated with its methylation status.3. The cells were treated with5aza-dC to demethylation, the expression of GPX3wasincreased, the cell proliferation and invasion capacity was decreased, and theapoptosis rate was increased.Conclusion:The methylation of GPX3gene promoter region led to its low expression in cervicalcancer and may play a very important role in the development of cervical cancer.