Abnormal Expression Of Runx2, Osterix In Renal Papillary Of Idiopathic Hypercalciuria Patients With Renal Calculus And Implications

ObjectiveTo explore the abnormal expression of osteoblast-like cell transcription factors Runx2, Osterix in idiopathic hypercalciuria (IH) patients with renal calculus and their effect on pathogenesis of stone formation in IH.MethodsScreen8patients with idiopathic hypercalciuria and with calculus (IH group), excluding all known secondary disease which could affect serum calcium or urinary calcium. Select8patients which need to be taken nephrectomy due to renal carcinoma or non-functioning kidney and precluding the cause of kidney stones(NC group).To take certain segments of renal papillary tissue under reasonable conditions in16patients.Real-time quantitative PCR and Western blotting were used to detect the protein and gene expression levels of Runx2and Osterix.ResultsThe mRNA expression of Runx2in IH group was1.437±0.115, while Runx2expression in the NC group was1.037±0.027, and expression values between the two groups was statistically significant (P<0.05);The mRNA levels of Osterix in IH group and NC group were1.826±0.462,1.030±0.072,respectively,and the two sets of data were significantly different (P<0.05).Osterix protein detected by Western blotting prompted the NC group and IH group were1.138±0.143and1.621±0.164,respectively, these was no significant difference between two groups,P>0.05. Runx2protein expression showed that the NC group was1.585±0.389, while the IH group was1.642±0.395, the difference was not statistically significant, P>0.05.ConclusionOverexpression of Runx2and Osterix mRNA in renal papillary of Idiopathic hypercalciuria (IH) patients with kidney stones indicates that it exists certain mechanism which have related to interstitial ectopic calcification, and the mechanism may be similar to the normal physiological processes of bone formation.