The Study Of Alteration Of Th1/Th2Cells Among Patients With Hepatitis E Virus

Objective：1. To investigate the alteration of Th1/Th2cell of patients with hepatitis e virus afteronset, that will be conductive to discuss preliminarily Cellular immune responseof patients with hepatitis e virus.2. To investigate the relation of the alteration of Th1/Th2cell of patients withhepatitis e virus and the virus itself, which will further realize liver damagemechanism of patients with hepatitis e virus.Methods：1.30anticoagulated peripheral blood and serum sample were collected from out-and in-patients with hepatitis e virus in the Department of Infection Diseases ofTongji hospital between June2010and June2011,and their biochemical indexwere collected together, such as ALT, TBil.2. The PBMCs were separated from the anticoagulated peripheral blood of Patientsin HE and the control group, and then were stimulated specifically by the restructuring ORF2protein（rORF2p） in vitro for6hours. Finally, the percentageof Th1/Th2cells in peripheral blood was detected by flow cytometry.3. The relative quantitative of HEV RNA in30serum sample were detected byfluorescence real-time polymerase chain reaction.Result：1. After the PBMCs were stimulated by specific protein, the percentage of Th1cellsamong30patients with hepatitis e virus and15controls was22.07%±8.45%,5.65%±2.83%, respectively. The HE group was significantly higher than thecontrol group. However, the percentage of Th2cells among the HE group and thecontrol group was2.12±0.85%，2.5%±1.25%, respectively. The differencebetween the two groups was no statistically insignificant.2. HEV RNA among8serum samples were detected for positive from30cases.According to whether HEV RNA was positive,30samples were divided into twogroups: group with HEV RNA, group without HEV RNA. The percentage of Th1cells and Th2cells among group with HEV RNA was25.50%±8.72%，2.17±1.02%, respectively. However，The percentage of Th1cells and Th2cells amonggroup without HEV RNA was20.82%±8.23%，1.98%±0.75%, respectively. Thedifference between the two groups was no statistically insignificant.3. The disease onset time of group with HEV RNA and group without HEV RNAwas6.9±4.0days，17.3±10.1days, respectively. The former was shorter than thelater according to statistics analysis, indicating the viraemia was short-lived.4. The TBil of group with HEV RNA and group without HEV RNA was110.4±69.1umol/L,225.7±126.1umol/L，respectively. The former was significantlylower than the latter. The fluctuation range of ALT between the two groups was879-2922U/L，48-5282U/L, respectively. Likewise, The former was significantlylower than the latter. Conclusion：1. Cell immunity of host was enhanced after infected with hepatitis e virus，performing Th1cells secreting IFN-γ were increasing reactively. It wasconsidered that Th1cells were involved in the infection and clearance of HEV.Nonetheless, the content of Th1cells among PBMCs was still not declinedobviously after clearance of HEV completely. It was considered that whether therewere yet immunogenicity materials of HEV.2. Liver cells were still further damaged after clearance of HEV completely, and itwas postulated that the imbalance of immune status caused the damage of livercells.