| ObjectiveTo observe the protective effect of Dendrobium Compound(DC) on the kidney of diabetic rats, and explore the pathogenesis of diabetic renal injury, revealing the mechanism of traditional Chinese medicine treatment on diabetic kidney damage.Methods60Sprague-Dawley female rats of SPF degree were randomly divided into2groups: normal control group and molding group. The normal control group was fed with basic diet, and the molding group was fed with high-saturated fat and sugar diet,6weeks, fasting for12h. The normal control group received a celiac injection of physiological saline, the molding group received a one-time celiac injection of streptozotocin (STZ25mg/kg), selected the rats in which fasting blood glucose was above16.7mmol/L as control group, then divided the control group into model group, low and high dose of DC groups and positive control group. All of the rats would be killed at the end of12th week, then detected relevant indexes by biochemistry, light microscopy, electron microscopy, immunohistochemistry. Blood glucose (FBG), total cholesterol (TC), triglyceride (TG), creatinine (Scr), blood urea nitrogen (BUN),24h urinary protein content (UAER), kidney weight index (KW/BW), renal tissue pathology and ultrastructure, renal transforming growth factor β1(TGF-β1) and vascular endothelial growth factor (VEGF) expression were observed.Results1.FBG, TC, TG:FBG, TC, and TG in DC of low and high dose groups were significantly lower than that of model group (P<0.01), FBG and TC in positive control group were lower than that of the model group (P<0.05or P<0.01); FBG and TG in DC of low-dose group were lower than that of the positive control group (P<0.05or P<0.01).2.BUN, Scr and24h urinary protein content:The intervention groups were significantly lower than the model group (P<0.01);3.KW/BW ratio:Compared to drug intervention in each group, KW/BW ratio was significantly lower in the model group (P<0.01); the DC high dose group was significantly lower than the positive control group (P<0.01).4.Renal tissue pathological changes:model group was more serious,while the DC low and high dose groups and the positive control group were mild. Both model group and treatment group are significantly higher than the normal group (P<0.01or P<0.05) in level of glomerular cross-sectional area, the average mesangial area (MMA) and the mesangial area (FMA). MMA and FMA in DC high dose group was significantly lower than the positive control group (P<0.05).5.Protein expression Of TGF-β1and VEGF:the model is significantly higher than the normal group (P<0.01), DC low and high dose groups and the positive control group were significantly lower than model group (P<0.01).Conclusion1.DC can reduce blood glucose, improve lipid metabolism, lower BUN, Scr, urinary protein and renal function.2.DC inhibit the overexpression of TGF-β1in diabetic rat kidney, affect ECM metabolism, improve renal morphology and slow down the process of renal fibrosis.3.DC inhibit the overexpression of VEGF in diabetic rat kidney, reduce proteinuria and delay the occurrence and development of DN. |
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