Experimental Study Of Effects Of Modified Guizhifuling Pill Combined With Wuling Powder On Rats With BPH

Objective:To observe the therapeutical effect of modified Guizhifuling Pill combined with Wuling Powder in treating Benign Prostatic Hyperplasia rat model.To investigate the mechanism of modified Guizhifuling Pill combined with Wuling Powder in order to provide experimental evidence for Chinese medicine on Benign Prostaic Hyperplasia(BPH).Methods:12male Wistar rats were chosen randomly as blank group, the rest60male Wistar rats after7days of modeling were randomly divided into model group、finasteride group、Chinese medicine low-dose group、Chinese medicine medium-dose group、Chinese medicine high-dose group.The model of BPH was established by injecting testosterone ProPionate after being eastrated.The groups received daily normal saline, finasteride,various doses of Chinese medicine respectively by intragastric administration.The blank group only received an injection of same amount of saline,while the rest60male Wistar rats were injected testosterone ProPionate for28days and then were killed for experiment. The weight and index of the prostate were measured.By means of immunohistochemistry,the expressions of Transforming growth factor β1(TGF-β1) were examined,By means of hybridization in situ, the expressions of Inducible nitric oxide synthase (iNOS) were examined.Results:①Compared with model group, the weight and index of the prostate of Chinese medicine groups decrease significantly (P<0.05or P<0.01).②Compared with model group, the expression of TGF-β1of Chinese medicine groups increase obviously (P<0.01).③Compared with model group, the expression of iNOS of Chinese medicine groups decrease significantly (P<0.05or P<0.01).④Compared with blank group,each index of model group had significant difference (P<0.01)Conclusion:①All of the Chinese medicine groups had obvious effect on BPH,and the high-dose one was best.②The mechanism may had some connection with inhibition of smooth muscle cells and tensile regulation of smooth muscle.③The basic pathogensis of BPH is related with kidney deficiency and stagnation of blood, we should treat the BPH by invigorating the circulation of blood、promoting diuresis、tonifying the kidney and activating Yang.