| Objective:Ovarian tumor is one of the most common malignant tumors of female genital organ. Due to the lack of early diagnosis, the ovarian cancer mortality rate ranks first in gynecological malignancies. Meanwhile, ovarian serous carcinoma (OSC) is the most common histological type of ovarian cancer, accounting for approximately40%of malignant ovarian tumors. Its recurrence rate and mortality rate are extremely high with the poor prognosis. In recent years, with rapid research and development of tumor pathology and molecular genetics, some scholars have made new progress in the cell origin and pathogenesis of OSC; they forwarded the dichotomy mechanism for the occurrence and development for OSC, i.e, OSC has two different tumor occurrence mechanism. Accordingly, some scholars put forward that the histology could be divided into two sub-systems, i.e, OSC could be categorized into high-grade and low-grade serous carcinoma. The two-tiered grading system complies with the dichotomy mechanism of OSC, which possesses the satisfactory application potential. The two-tiered grading system could interpret why there exists significant difference in histological features, immunophenotype, the response to chemotherapy and prognosis in serous carcinoma. Meanwhile, The two-tiered grading system could be utilized to guide the early screening and clinical treatment and assess the prognosis. As for the differences of histological features and molecular performance in high-grade and low-grade serous carcinoma, there are numerous researches while there are few reports on the research on the immuno-histochemical method to compare the protein perforamnce difference of high-grade and low-grade serous carcinoma; This experimental mainly study the immuno-histochemistry performance and investigate the implication of P53,CyclinDl and P16in high-grade and low-grade serous carcinoma.Methods:45cases of OSC are collected in2010-2011from Tianjin Central Hospital of Gynecology Obstetrics. These OSC cases are divided into two groups:19cases of low-grade serous carcinoma and26cases of high-grade serous carcinoma.25cases of borderline ovarian serous cystadenoma (SB) and21cases of ovarian serous cystadenoma (SA) are chosen for comparison. The immune-histochemistry S-P is utilized to test the performance of P53, CyclinDl and P16in4sampled groups of tissues. The statistical results are analyzed for the correlation by SPSS16.0to discuss the performance implication.Results:The positivity of P53in the low-grade carcinoma is5%while that in high-grade carcinoma is69%. The difference is statistically significant (P<0.05); the positivity of CyclinDl in the low-grade carcinoma is89%while that in high-grade carcinoma is15%. The difference is statistically significant (P<0.05);The positivity of P16in LGSC is84%while that in HGSC is92%. The rate in high-grade carcinoma is apparently higher than that in the low-grade carcinoma. While P>0.05, the difference is not statistically significant. The positivity performance of P16in the benign, borderline and malignant tumor groups are24%,72%and89%respectively. Between the benign tumor, borderline and malignant group difference was statistically significant (P<0.05). The positivity performances of P53in benign, borderline and HGSC are5%,4%and69%respectively.The difference between the benign, borderline tumor group and HGSC are statistically important (P<0.05). The positivity performances of CyclinDl in the benign, borderline and LGSC are10%,64%and89%. The differences between benign, borderline and LGSC tumor groups are statistically significant (P<0.05).Conclusion:The positivity of P53more performs in high-grade OSC while the positivity of CyclinDl more occurs to SB and low-grade serous tumors. The positivity of p16more performs in SB, OSC and serous ovarian cancer tissue without pelvic lymph node metastasis. The ovarian HGSC and LGSC have different pathogenesis. The immuno-histochemical staining results of P53and CyclinDl contribute to the dichotomy classification of OSC. |
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