Studies On Relaptionships Among Scavenger Receptor Class B Type â…  Polymorphisms, Lipid Metabolism And Coronary Heart Disease

Objective:With the development of economy and people’s lifestyle change, Coronary heart disease (CHD) is one of the leading causes of death worldwide. It is a complex disease resulted from numerous gene-gene and gene environment interactions. It is commonly accepted that dyslipidemia, including elevated levers of atherogenic lipoproteins and/or reduced levels of high density lipoprotein, is a prerequisite for most forms of CHD. Moreover, epidemiological studies have revealed that multiple genes, especially those involved in lipid metabolism. High density lipoprotein (HDL) is a well-known anti-atherosclerotic factor, which exerts the activity of anti-atherosclerosis through reverse cholesterol transportation. Scavenger receptor BI is a receptor of HDL, participating reverse cholesterol transportation. Singer nucleotide polymorphisms (SNPs) possess the characteristics of large numbers and dispersed distribution. SNPs, especially those in coding regions and gene regulatory elements are implicated as a causative factor in human genetic disease. So far, the association of scavenger receptor class B type I (SR-BI) polymorphisms on serum lipids, lipoproteins, apolipoproteins variation and its relationship with coronary heart disease focused on the exonl、Sand intron5. On the study of SR-BI SNP,there are more common in Europe and the United states,relatively few studies on the Chinese Han population. Ethnic differences, the study of foreign SR-BI gene polymorphism may not apply to the Chinese people, we further our understanding of the possible role played by SNP of the SR-BI gene in dyslipidemia and CHD.Elaborate the association of scavenger receptor class B type I (SR-BI) polymorphisms on serum lipids, lipoproteins, apolipoproteins variation and its relationship with coronary heart disease in Chinese Han crowd.It will provide a new strategy for the prevention of the hard of atherosclerosis disease.Methods:We selected513examples Han nationality people in China undergoing coronary angiography in Tianjin Chest hospital in October2010to October2011continuously,With case-control study, We obtain clinical information (age、gender、 height、weight、hypertension diabetes、smoking), and detect objects lipid^blood glucose. All examples were grouped into370cases and143controls with PCR-RFLP analyzing the SR-BI genotypes then studied relaptionships among scavenger receptor class B type I polymorphisms, lipid metabolism and coronary heart disease.Results:(1) Tere are GG、GA、AA genotypes of the SR-BI exon1gene, Intron5only has a CC genotype, Exon8gene have CC、CT、TT genotypes of Han nationality.(2) The GA+AA genotype frequency and A allele frequency of SR-BI exonl in the CHD group had no significant difference with those in control group(P>0.05). The CT+TT genotype frequency and T allele frequency of SR-BI exon8in the CHD group had no significant difference with those in control group(P>0.05).(3) The serum level of HDL-C and ApoAlin SR-BI exonl gene GA+AA genotype of male subgroup was markedly higher than in GG genotype subgroup of the same group in CHD group (P<0.05).(4) The serum level of LDL-C and Lpa in SR-BI exon8gene CT+TT genotype of female subgroup was markedly higher than in CC genotype subgroup of the same group in control group (P<0.05).(5) Compared with SR-BI exonl gene GA+AA genotype subgroup and GG genotype subgroup, Compared with of SR-BI exon8gene CT+TT genotype subgroup and CCgenotype subgroup, gensini score had no significant difference(P>0.05).(6) Logistic regression analysis showed that there was no significant difference among the exonl(G→A), exon8(C→T) and coronary heart disease(P>0.05).Conclusions:1、The exon1、8polymorphism of SR-BI gene is no association with coronary heart disease of Han population in Tianjin area.2、A allele of SR-BI exonl is possibly associated with the metabolism of HDL and ApoAl of male. HDL and ApoA1have the anti-atherosclerotic effection, speculate that the SR-BI gene exon lmutation maybe a protective factor for CHD.3、T allele of SR-BI exon8is possibly associated with the metabolism of LDL and Lpa of female. LDL and Lpa have the atherosclerotic hardening effection, speculate that the SR-BI gene exon8mutation maybe a risk factor for CHD.4、The exon、8polymorphism of SR-BI gene is no association with CHD.