Expression In A Timely Manner Relative To Research Of GAP-43，eNOS，HIF-1α After Primary Brain Stem Injury In Rats

Objective To investigate the organization after the primary brain stem injuryin rats Expression of GAP-43,eNOS,of HIF-1α in its expression changes withdifferent injury time.Methods: SD rats75,1h after the impact method toproduce animal models of rat primary brain stem injury,collecting damage14dwithin nine different time points in brain stem,and normal control group,shamgroup and after the death of the injury group.By immunohistochemical SPmethod detection of GAP-43,eNOS and HIF-1α expression changes atdifferent time points.Results The expression after3h in brain steminjury,increased expression of the normal control group,the sham group,andpost-mortem injury group were statistically significant of GAP-43positive(P<0.05).7d reached a peak and the normal control group,sham group, afterthe death of the injured group and neighboring group was statisticallysignificant (P<0.05).14d dropped to normal,compared with normal controlgroup, sham group and the death of injured group was not statisticallysignificant (P>0.05);expression of eNOS was increased1h after injury,withthe normal control group,sham group and injury group after death comparisonwas statistically significant (P<0.05).expression of9h to peak, and the normalcontrol group,sham group, after the death of the injured group andneighboring group was statistically significant (P<0.05),7d expression tonormal,and normal control group, sham group and post-mortem injurygroup,no statistically significant (P>0.05);of HIF-1α3h after injuryexpression is increased,and the normal control group, sham group and afterthe death of the injury group was statistically significant (P<0.05),24hexpression to peak, and with the normal control group,sham group, after thedeath of the injury group and neighboring group was statistically significant(P<0.05),7d decreased to normal, and normal control group,sham group and post-mortem damage group was not statistically significant(P>0.05).Conclusion: The rat brain stem injury,GAP-43,eNOS,HIF-1αexpression are raised each were some timing variation is expected to provide areference for the forensic brain stem injury in the early time and the time afterthe death of inference.