The Influence Of1,25-dihydroxyvitamin D3on Renal Expression Of TGF-β1and CD68, MCP-1in Type2Diabetic Mellitus Rat

Objective: By exploring the effects of1,25-dihydroxyvitamin D3on renalexpression of transforming growth factor-beta1(TGF-β1) and CD68, monocytechemoattractant protein-1(MCP-1) in type2diabetic mellitus(T2DM) rats, this paperdiscusses the influence of1,25-dihydroxyvitamin D3to T2DM rats，kidney. Methods:The experiment was divided into two stages: prevention and treatment.60Sprague-Dawley rats were randomized into3groups: normal control rats(gavaging equalpeanut oil), T2DM(gavaging equal peanut oil) rats and T2DM rats intervented with1,25-dihydroxyvitamin D3(gavaging1,25-dihydroxyvitamin D3).The remaining rats of theprevention stage were divide into normal control rats,diabetic nephropathy(DN) rats andDN rats treated with1,25-dihydroxyvitamin D3. T2DM rats and T2DM rats interventedwith1,25-dihydroxyvitamin D3feed with high fat and high sugar feeding. Six weeksafter, this two groups iv injection small dose STZ.The T2DM rats test trace protein withurine dipstick for rats morning urine three times or more after injection STZ, if positive, itis Type2diabetic nephropathy rats and measure24hours proteinuria continuely. After8weeks and15weeks,kidney tissues morphology and kidney function,24-hour urinaryprotein quantitative index change were measured after15weeks of treatment, whileTGF-β1and CD68, MCP-1expression in kidney cortex were observed byimmunohistochemistry. Results: In prevention stage, Comparing with normal controlgroup, triglycerides, blood sugar(BG),24-hour urinary protein and cholesterol (CHO)were higher in T2DM group,but weight and urea nitrogen (BUN)was less in it.24-hoururinary protein and cholesterol (CHO)were higher in T2DM group than the interventiongroup.24-hour urinary protein and BG in the intervention group were higher than normal control group, but BUN is less than normal control group; In the intervention group theweight of kidney and weight of rat were also higher than T2DM group,but CHO and24-hour urinary protein were less than T2DM group. The expression of TGF-β1、MCP-1and CD68in T2DM group were more than the other groups.In treatment stage, serumcreatinine(Cr), weight, BG and CHO, TGand24-hour urinary protein quantitative weresignificantly higher in the DN rats than those in the normal control rats (P＜0.05). Theexpression level of CD68, MCP-1, TGF-beta1and triglyceride level in the1,25-dihydroxyvitamin D3treated group were obviously lower than those in the DN rats(P＜0.05). Conclusion:1,25-dihydroxyvitamin D3can inhibit the expression level ofTGF-beta1, MCP-1etc of diabetic rats kidney and restrain macrophages in order to protectkidney of diabetic rats.