The Expression Of Wnt5a In Breast Cancer Cell Line MCF-7and The Correlation With E-cadherin And Vimentin Expression

Objective: In this study, we have performed an analysis of the expression of wnt5a、E-cadherin and vimentin in breast cancer cell line MCF-7and the normal human mammary epithelial cell line MCF-10A.Methods:MCF-7and MCF-10A cells were divided into three groups randomly, the expression of wnt5a、E-cadherin and vimentin used by RT-PCR respectively and performed an analysis of the correlation.Results:1.The expression rate of wnt5a in cell line MCF-7(16.93±2.97%) was significantly lower than that in cell line MCF-10A (27.47±2.76%)(P<0.05).2.The expression rate of E-cadherin in cell line MCF-7(12.97±1.35%) was significantly lower than that in cell line MCF-10A (20.43±2.60%)(P<0.05).3.The expression rate of Vimentin in cell line MCF-7(16.53±0.85%) was significantly higher than that in cell line MCF-10A (6.33±2.08%)(P<0.05).4. Both the expression of Wnt5a and E-cadherin in cell line MCF-7were lower than that in cell line MCF-10A. The expression of Wnt5a and E-cadherin in cell line MCF-7and cell line MCF-10A were positively related (rs=0.997, P<0.05). The expression of Vimentin in cell line MCF-7was higher than that in cell line MCF-10A. The expression of wnt5a and Vimentin in cell line MCF-7 and cell line MCF-10A was inversely related (rs=-0.998, P<0.05).Conclusions:1. The expression of Wnt5a in human breast cancer cell line MCF-7was significantly lower than in the normal breast epithelial cell line MCF-10A. These data indicate that wnt5a may act as a tumor suppressor gene in breast cancer.2. The expression of Wnt5a in human breast cancer cell line MCF-7and E-cadherin expression were positively related, with the expression of vimentin was negatively related. These results suggested that Wnt5a may influence invasion and metastasis of breast cancer cell, through the breast epithelial mesenchymal transitions.