Fabrication Of Electrospun Fiber Mat And Its Effect In Mice Skin Wound Protection

Purpose of research:Skin burns or chronic skin wounds with varying degrees of skin missing, usually experienced slow wound healing process due to the lack of wound protection, and those could lead to poor quality of skin wound healing. Therefore, optimal wound dressing materials which could promote skin regeneration are needed in the clinical. Currently wound dressings materials could be divided into three categories:natural materials, animal tissue derivatives products, and synthetic polymer materials. The natural materials dressings and animal tissue derivatives products dressings had certain disadvantages, like lack of sources、difficulty in storage. The synthetic polymer materials were mainly composed of synthetic polymers and bioactive molecules, and displayed excellent mechanical properties as well as great biocompatibility. Polycaprolactone (PCL)-gelatin fiber mat was a porous biomimetic stent with high porosity and high specific surface area and considered as a new generation of artificial dressing. Moreover, it could deliver growth factors into wound area as a drug delivery device to promote wound healing. The aim of the project was to fabricate bioactive mats for wound dressing by electro-spinning technique based on PCL and gelatin, and then assess the biocompatibility of electrospun fiber mats both in vitro and in vivo.Research approach:1. BMSCs (human bone marrow mesenchymal stem cells) were isolated and characterized in terms of osteogenic differentiation potential.2. Both PCL and PCL-gelatin mats were fabricated through electrospinning technique, and BMSCs were seeded on mats and their in vitro biocompatibilities were characterized by the CFDA staining, MTT assay.3. Based on the Kunming mouse wound model, different mats were applied on the wound and the wound healing process was observed through calculation of the area of the wound over the time and histological section analysis by H/E staining.Result of research:1.Uniform PCL mats could be harvested under solute mass fraction of15%, spray voltage of10kv, speed of solution flow of0.75ml/h, distance between the needle plate of15cm; uniform PCL-gelatin mats could be harvested under PCL solution (mass fraction of12.5%) and gelatin solution (mass fraction of2.5%) volume ratio of4:1, spray voltage of10kv, speed of solution flow of0.75ml/h, distance between the needle plate of15cm.2. At the first day after seeding, BMSCs on PCL-gelatin mats performed better adhesion to the surface with pseudopods number more than3, while cells on PCL with less than3pseudopodia; On19th day, cells on PCL-gelatin mats reached the growth plateau earlier than the cells of the PCL mats, but later than the blank control group (12-well culture plate).3.Animal experiments show that PCL, the PCL-gelatin and aFGF treatment groups presented more wound healing area with more hair follicles formation and collagen deposition than blank control group on11th day.