The Correlation Of Chronic Aplastic Anemia Chinese Dialectical Typing And Peripheral Blood Teiomerase Reverse Transcirptase (HTERT) MRNA Expression Levels

Objective: Determination of Chronic aplastic megaloblasts in eripheral bloodmononuclear cell telomerase reverse transcriptase （hTERT） mRNA expression levels，In prder to Explore the correlation of CAA TCM dialectical type and peripheral bloodmononuclear cells of hTERT mRNA expression levels，Further investigate the the essenceof the CAA from molecular and try to seek new treatment ideas.Method:30patients with CAA were grouped in accordance with the ChineseMedicine of the Standard,“Shen yang xu group”10cases,“Shen yin xu group”10cases,“Shen yin yang liang xu group”10cases, Determination of30cases of CAA in patientswith Peripheral blood mononuclear cells of hTERT mRNA expression using Real-timequantitative PCR method,and selected10cases of normal physical examination as acontrol group. Observe the correlation of CAA TCM dialectical type and peripheral bloodmononuclear cells of hTERT mRNA expression levels; Observe the correlation ofperipheral blood mononuclear cells of hTERT mRNA expression levels and CAA in patientage, gender and the Reduce degree of White blood cells, red blood cells and platelets;Observe the correlation of CAA TCM dialectical type and CAA in patient age, gender andthe Reduce degree of White blood cells, red blood cells and platelets;And Statisticalanalysis of the results, To study their correlation.Results:（1）Peripheral blood mononuclear cells of hTERT mRNA expression levelsof CAA patients in “Shen yin xu group”,“Shen yang xu group”and “Shen yin yang liangxu group”, Between the two groups no significant difference （P>0.05）; Comparisonwith the normal control group, The peripheral blood mononuclear cells of hTERT mRNA expression levels of CAA patients in“Shen yin yang liang xu group”are lower, Therearesignificant differences （P <0.05）;（2） Group CAA patients by age, The peripheralblood mononuclear cells of hTERT mRNA expression levels of CAA patients>40-year-oldgroup is lower than CAA patients≤40-year-old group and normal control group. Therewere significant differences （P <0.05）. The peripheral blood mononuclear cells ofhTERT mRNA expression levels of CAA patients≤40-year-old group, normal control≤40-year-old group and normal control>40-year-old group，There are no significantdifferences （P <0.05）.（3） Peripheral blood mononuclear cells of hTERT mRNAexpression levels of CCAA patients, the comparion Male group and female group is nosignificant difference （P>0.05）.（4） CAA patients were divided into ever two groupsaccording to blood leukocytes, hemoglobin, platelet count，Peripheral blood mononuclearcells of hTERT mRNA expression levels of ever two groups,there are no significantdifferences（P>0.05）（.5）The age of “Shen yin yang liang xu group”is older than “Shenyin xu group”and “Shen yang xu group”,there is significant difference（P <0.05）. The ageof “Shen yin xu group”“Shen yang xu group” and Normal control group, comparedbetween the two groups there are no significant differences（P>0.05）（.6）The gendercomposition of “Shen yin xu group”,“Shen yang xu group”and “Shen yin yang liang xugroup”compared with normal control group, there is no significant differences （P>0.05）.（7） The peripheral blood white blood cells, hemoglobin, platelet count of CAApatients in “Shen yin xu group”,“Shen yang xu group”and “Shen yin yang liang xugroup”, compared between the two groups there are no significant differences （P>0.05）.Conclusion: Peripheral blood mononuclear cells of hTERT mRNA expression levels ofCAA patients in “Shen yin yang liang xu group”is low,the peripheral blood mononuclearcells of hTERT mRNA expression level of CAA patients>40-year-old group islow,Show that Peripheral blood mononuclear cells hTERT mRNA expression level andtelomerase activity reduced may be one of a Molecular Biology Pathogenesis of CAApatients in “Shen yin yang liang xu group”and>40-year-old group. CAA patients in“Shen yin yang liang xu group”with clinical features of older, Confirmed the TCMargument that“Into middle age, kidney failure since”. Speculate that Peripheral bloodmononuclear cells hTERT mRNA expression level and telomerase activity reduced may be one of the the manifestations of Molecular Biology of “Shen xu”.