The Study Of Deep Cerebral Veins After Subarachniod Hemorrhage

Objective: To observe the deep cerebral vein after subarachnoid hemorrhage in therabbit model with MRI. Through this study may provide a theory basis of the cerebralvasospasm treatment after subarachnoid hemorrhage.Methods:52New Zealand rabbits were randomly divided into subarachnoidhemorrhage group (43) and saline group (9). The SAH animals were made by infusingthe autologous arterial blood into the cisterna magna twice, and the normal saline (NS)group had saline instead of blood.14SAH rabbits were sacrificed and observed bymicroscopic anatomy. The animal behavior and neurological function of4rabbits inSAH group and2rabbits in NS group were observed. Magnetic resonance scan wasperformed before making model and5day after making model again in15rabbits inSAH group and3rabbits in NS group to observe whether the internal cerebral vein aschange as basilar artery. The internal cerebral vein, basilar vein and basilar arterydiameter changes were observed by MRI before modeling(0d) and after modeling (1,3,5,7,9,11d) with10rabbits in SAH group and4rabbits in NS group; Spasm degree=(diameters of0d–diameters after modeling)/diameters of0d; Rate=(previousdiameter-after time diameter)/previous diameter.Results:①Microscopic anatomy showed that black color coagulated blood wasobviously found around the ventral surface of brain stem, lateral ventricles andquadrigeminal cistern.②Animal behavior observation: NS in the groups of animals in1d appetite a slight decline, from cleanliness is a bit poor, did not appear limb paralysisand neural function damage, after1d completely normal. SAH group rabbit immediately appear nystagmus, breathing added fast, limb and stiff neck, muscle finequiver. Symptoms in the second note blood after5d heaviest,7d~9d ease and recovergradually,11d animals returned to normal.③Deep cerebral vein MRI observation: InNS group, no statistical difference was found (P>0.05) between the diameters beforeand after making model of internal cerebral vein, basilar vein and great cerebral vein. InSAH group, the diameters of internal cerebral vein, basilar vein, great cerebral veinwere separately (0.57±0.13) mm、(0.38±0.08) mm、(1.33±0.31) mm before makingmodel. However, the diameters of them were (0.42±0.08) mm、(0.25±0.12) mm、(1.26±0.31) mm at5d after making model, and there were statistical differencesbetween the diameters before and after making model of internal cerebral vein and greatcerebral vein.④Vascular diameter variation rules of magnetic resonance measurement:In NS group, no difference was found. In subarachnoid hemorrhage group, The meandiameter of internal cerebral vein was obviously narrow after making model in1d,eased after the3d, and reached a peak around5~7d, and gradually recovered during9~11d. The diameter of basilar vein was also appeared obviously narrow after makingmodel in1d, in3d was no better, and then the change rule was similar to internalcerebral vein, reached a peak around5~7d, and gradually recovered during9~11d. Thebasilar artery’s diameter had no significant changes after making model in1d. Itreached the peak in3~5d. The spasm gradually eased from7d and recovered in11d.⑤The narrow degree of vein and artery: The narrow degree of internal cerebral veinwas18.86%in1d;13.21%in3d; in5d was33.96%; the narrow degree eased from7dto11d, and in11d was3.77%. The basilar vein’s narrow degree was18.42%in1dafter modeling;23.68%in3d;34.21%in5d; in7d to11d, the narrow degree wasrecovered, in11d it was5.26%. In basilar artery, the narrow degree was3.03%;36.36%in3d;33.33%in5d; it was recovered in7d to11d, the degree were13.64%,9.09%and0%.⑥The change rate of vein and artery: In1d, the change rate of internalcerebral vein was reduced18.89%than0d; in3d it was increased6.98%than1d; and in the follow days, the change rates were23.91%,-8.57%,-18.42%and-13.33%. Thechange rate of basilar vein in1d was18.42%; in3d was6.45%; changed in5d was13.79%; and then the change rates were-8.00%,-11.11%and-20.00%. The change rateof basilar artery was only3.03%; in3d the change was greatly to34.38%; then in thefollow days, the change rates gradually expand, it in turn were-4.76%,-29.55%,-5.26and-10.00%.Conclusions:①After subarachnoid hemorrhage, also be able to reach the brainhemorrhage deep vein area;②The animal behavior not only the most serious periodbasal veins appear spasms, the brain also has deep vein stenosis;③The internalcerebral vein appears narrow but the rules is different of basal artery;④The vasculardiameter variation rules and the narrow degree shows that the narrow contraction for theinitiative. Various vasospasm of deep cerebral veins after SAH could appear. But spasmof the time in deep cerebral vein and basilar artery has apparent difference.