| In recent ten years, the detection and the treatment of cancer have being advanced rapidly, thereby, the patients have a longer living time than ever. However, the quality of patient’s life was affected seriously by the cancer pain. Patients with advanced tumor always suffer from a serious cancer pain. And the cancer pain has been paid extensive attention because it decreases the quality of patient’s life, disrupts the therapy schedule and shortens patient’s living time. The main reason of cancer pain is the bone metastasis and the soft tissue infringement of tumor, so it is very important to establish the animal model of cancer pain. In1999, Schiwei reported a femur cancer pain model in mice with NCTC2472fibrosarcoma cells. In2001, Wacnik reported a calcaneus cancer pain model in mice. And in2002, Medhurst reported the first tibial cancer pain model in rats with MRMT-1mammary gland carcinoma cells. Following, there are a series of bone cancer pain models, and the emergency and development of these models enhanced our knowledge of the mechanism of cancer pain generate and maintenance, and led to the development of novel therapeutic approaches. In our study, we choose MADB-106mammary gland carcinoma cells to establish the tibial cancer pain model in rats, evaluate the hyperalgesia behavior, monitor the destruction of bone by X-ray and histology, and analyze the expression of GFAP in spinal by immunofluorescence.MethodsMADB-106mammary gland carcinoma cells were inoculated in the marrow cavity of SD rats’tibia. Spontaneous pain was assessed by the frequency of spontaneous paw withdraw, move-evoked pain was observed by the extent of hindlimb claudication when the rats walked. The structural damage of the bone was monitored by X-ray and histology. The activations of the spinal astrocytes were detected via the expression of GFAP by immunofluorescence.Results1. Velocity changes The velocity of two groups rats have no significant difference about found level and8d post-inoculation.level. The growth velocity of rats was much lower in M group than in C group since15d post-inoculation.2. Frequency of spontaneous paw withdraw The frequency of spontaneous paw withdraw was much more in M group than in C group since8d post-inoculation, but there is no significant difference. Since15d post-inoculation, the frequency in M group of rats significantly increased than in C group of rats.3. Move-evoked pain score The move-evoked pain score of the M group was significantly higher as compared with C group since15d post-inoculation.4. Image changes of bone8days post-inoculation with MADB-106cells, the tibia of rats showed signs of small radiolucent lesions. By day14after inoculation, some loss of medullary bone and destruction of cortical bone were observed apparently.22days after inoculation, major cortical destruction of was detected.5. Histology changes The histology of the bone showed the bone marrow cavity was full of tumor cells and the cortical bone had been destroyed in M group of rats.6. Immunofluorescence of astrocytes GFAP staining of the spinal cord showed increased staining, and GFAP stained astrocytes were hypertrophied in M group of rats. ConclusionsMADB-106mammary gland carcinoma cell is a good choice to build a rat bone cancer pain.Imageology and histology analysis show that the pain progressively becomes heavy with tumor cells growth and bone destruction.1. The hyperalgesia in rats is developed two weeks after inoculation of MADB-106mammary gland carcinoma cells. When the hyperalgesia occurs, the expression of GFAP is increased and the astrocytes are hypertrophied. These results indicate that the development of hyperalgesia may be related to activation of spinal astrocytes... |
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