Enhanced Adhesion And Invasion Of Porphyromonas Gingivalis Andactinobacillus Actinomycetemcomitans To Epithelial Cells By Fusobacterium Nuleatum

Chronic periodontitis is one of the major causes of tooth loss in adults, affecting health and well being. In china, it has a high incidence. Now periodontitis is considered as a potential risk factor for the development of some systemic diseases and manifestations such as cerebro-vascular and cardio-vascular diseases.Chronic periodontitis is a bacterially induced inflammation of the periodontium. Fusobacterium nuleatum has been considered as a key pathogen in chronic periodontitis and it could enhance the abilities of other periodontal pathogens to adhere epithelial cell. Objective:To investigate the abilities of adhering and invading epithelial cells of p. gingivalis and A. actinomycetemcomitans when mixed with F nucleatum.Methods:2×105CFU/ml pure bacterial suspension was prepared with the tested strains p. gingivalis ATCC33277、A. actinomycetemcomitans ATCC29523、F. nucleatum ATCC25586and different combination of three bacterial. The suspension added to the well with Ca9-22cells to incubate in air with5%CO2.at37℃. The adhesion and invasion of the bacterial to Ca9-22cells were assayed. One-way AVONA analysis was used to study the bacterial abilities of adhering and invading epithelial cells of Porphyromonas gingivalis、 Actinobacillus actinomycetemcomitans.RESULTS:The adhesion and invasion of P.gingivalis and A actinomycetemcomitans to Ca9-22cells were enhanced two to four times by F. nucleatum.CONCLUSION:The adhesion and invasion of P.gingivalis and A actinomycetemcomitans to epithelial cells were enhanced by F. nucleatum.