The Effects And Mechanisms Of Phlorizin On Mice Liver Fibrosis Induced By S.Japonicum

Phloridzin is a flavonoid glycoside, which is rich in the apple bark, roots and other organs. The effect of Phloridzin on anti-hepatic fibrosis in mice showed that Phloridzin significantly reduced serum levels of ALT, AST, HYP and liver index. Histopathological observations showed that the four flavonoids could ameliorate the liver fibrosis in different degree. Phloridzin had effect on preventing hepatic fibrosis. To study the effect and mechanisms of each dose phloridzin treating on schistosomiasis liver fibrosis and find an effective drug concentration for anti-schistosomiasis liver fibrosis.Methods:After one week adaptive feeding and with20Kunming mices as normal group, the other180were induced liver fibrosis model by Schistosoma japonieum. All the infected mice except the mode group were orally insecticidal by Praziquantel500mg/kg for two days after infected6weeks. Then they were divided into seven groups.①Model group(N=20): gavaged with physiological saline from7th to15th week;②Praziquantel group:gavaged with physiological saline7th to15th week;③Interferon gamma group:injected in cervical subcutaneous by interferon gamma500iu/kg/d from7th to15th week;④Phloridzin groups: gavage with10mg/kg/d,20mg/kg/d or40mg/kg/d Phloridzin from7th to15th week;⑤Nomal group:20nomal mices were gavage with0.5% NacL10ml/kg7th to15th week. All mices were treated after infected6weeks and sacrificed12hours after drug treatment for15weeks.The mices serum were collected for further biochemical detection of Hyp, HA, PIIINT, LN, TGF-β1and PDGF-BB. Histological specimens were stained with HE and studied microscopically to observe the pathologic changes of liver fibrosis. Expression of TGF-β1and PDGF-BB was detected by immunohistochemical technique and multimedia color pathographic analysis system.Results:The content of Hyp, HA, PⅢNT, LN, TGF-β1and PDGF-BB tended to decrease from4th week to8th week gavaging Phloridzin, and the various indicators in high doses of Phloridzin were the closest to the blank group and inhibited Schistosomiasis liver fibrosis significantly. The results indicated that Phloridzin showed dose-effect enhanced relationship. HE staining solution showed liver degeneration and necrosis were obvious reduced and the lobular structure is the most complete degeneration in high doses of Phloridzin group. TGF-β1and PDGF-BB in high doses phloridzin group contented with the control group, the praziquantel group, low and middle dose phloridzin group had a significant difference (P<0.01) and were not significant difference between interferon gamma group and Phloridzin groups (p>0.05) Compared with model group, interferon gamma group and Phloridzin groups were signigicantly decreased(P<0.01). There were signigicantly differences between low and middle dose group and interferon gamma group (P<0.05). There were signigicantly differences among each dose phloridzin group (P<0.05)Conelusion:(1)Praziquantel can not alleviate liver fibrosis induced by Schistosoma japonicum.(2)Based on the use of Praziquantel killing Schistosoma japonicum, treatment with Phloridzin could reduce the fibrogenic factor TGF-β1and PDGF-BB, alleviate liver inflammation, protect liver cells, reduce fibrosisi induced by Schistosoma japonicum and reduce the expression of collagen fibers.(3)Phloridzin (40mg/kg/d) could inhibite schistosomiasis liver fibrosis most and showed dose-effect enhanced relationship.