Linaoxin Capsule Secondary Development Of Pharmacodynamic

Linaoxin Capsule (LNXJN) is the sale of traditional Chinese medicine preparation whichproduction by Jilin Yanbian Aodong Pharmaceutical. It has the effects of “Promote blood flowand Remove Stasis, Smooth breathing and Remove Phlegm, Dredge Meridians and Relievepain”. Commissioned by the enterprise, we carried out related research and exploration toexpand and explore the clinical use and acting mechanism of the drug.Purpose: The subject of this study is through the animal models of cerebral ischemia,myocardial ischemia and hypertension, combine physiological and biochemical characteristics,compare the changes of biochemical indicators in plasma and tissue, to provide theexperimental basis for clinical rational drug use, provide related theoretical basis for thefurther expansion of its clinical indications, and in order to provide new method and newideas for the secondary development of the listed traditional Chinese medicine.Method:1、Established focal cerebral ischemia model by suture method, observe thedamage of brain tissue of rats by HE staining method, detect the content of SOD and MDA inthe plasma and brain tissue by colorimetry, to observe the therapeutic effect of LNXJN onfocal cerebral ischemia.2、Established experimental myocardial ischemia rats model by themethod of ligature left anterior descending of the coronary artery. Observed the damage ofvascular and myocardial tissue by HE staining method, detect the content of TXB2and6K PGF1αin plasma by enzyme linked immunosorbent assay (ELISA), to observe thetherapeutic effect of LNXJN on myocardial ischemia.3、 Established experimentalhypertension model by the method of ligature left renal artery and measuring the systolicblood pressure of rats to observe the impact of LNXJN on hypertension model.4、Measuringthe diameter of mouse ear micro vascular and blood flow velocity by the experiment of mouseear microcirculation, to observe the impact of LNXJN on peripheral circulation.5、To observethe sedation effect of LNXJN by the experiment of spontaneous activity.6、To observe theanalgesic effect of LNXJN by hot plate test.Result:1、Each dose group of LNXJN and positive control group can increase theactivity of SOD and reduce the content of MDA in plasma and tissue of focal cerebralischemia model rats, compared with model group, there are significant differences (p<0.05). 2、Each dose group of LNXJN and positive control group can reduce the concentration TXB2and increase the concentration of6k PGF1αin plasma of myocardial ischemia model rats,compared with model group, there are significant differences (p<0.05).3、Compared withmodel group, hypertension model has no significant differences on blood pressure of eachtreatment group (p>0.05).4、The middle dose group of LNXJN and positive control group canexpansion micro vascular diameter, compared with the control group, there are significantdifferences (p<0.05), the middle dose group of LNXJN and positive control granules groupcan promote blood flow, compared with the control group, there are significant differences(p<0.05).5、Compared with control group, each dose group in LNXJN can make spontaneousactivity had significantly fewer, the middle dose group is the best(p<0.01), compared with thepositive control group, significant differences(p<0.01).6、Compared with control group, themiddle dose group of LNXJN and positive control group make mice licking feet timesignificantly prolonged2h after medication, indicated LNXJN have significant analgesiceffect.Conclusion: LNXJN can enhance the activity of SOD and reduce MDA content in ratplasma and brain tissue of focal cerebral ischemia model, and therefore it have protectiveeffect in rat brain damage caused by cerebral ischemia. LNXJN can enhance the6k PGF1αcontent and reduce TXB2content in rat plasma and brain tissue of myocardial ischemia model,and therefore it have a good therapeutic effect on myocardial ischemia model in rat. LNXJNcan expand capillaries and promote blood flow, and it also has a phase auxiliarypharmacodynamic of sedative and analgesic.