| According to the World Health Organization, there are about10%~15%marriedcouples are diagnosed as infertility which is in part due to male facts. The infertilitywhich is cause by male facts is called male sterility. Out of those causes of maleinfertility, dyszoospermia is the most common reason. And the most probableperformance of primary sterility is on refined and less refined disease.Dyszoospermia is a complex disease, which is cause by genetic factor andenvironmental factor. Though under the same environment, the individual symptomswith different genetic are vary from unit to unit. Genetic factor plays an important partin dyszoospermia. At present, scientists have made great progress in the geneticetiology of dyszoospermia and found some genetic factors which lead todyszoospermia, such as the shortage of the chromosome Y, chromosomal variation,deletion and mutation of some genes. But these known genetic causes only canexplain a handful of male sterility cases, and there are some causes of male infertilityare waiting to be clarified.Human sperm production is an extraordinarily complex process. It involves inthree main steps, including cell proliferation, meiosis and cell differentiation. In theprocess of the formation of the sperm, any abnormal related gene in the abnormal canblock sperm production。 Therefore, those genes that play important roles in spermproduction are recognized as main candidate genes of male infertility. With thedeepening of the research, many relevant genes have been found during spermproduction, but it needs more time to study whether they are related with spermproduction.Gene GSTM1and gene GSTT1are two genes that we have paid close attention toin recent years. Protein digestion of gene GSTM1and gene GSTT1can protect sperm production, and they are in importance during the detoxication of endogenoussubstances and something foreign. Since the above two genes are deletion polymorph,the polymorphism can lower the enzymatic activity survival. Research shows theshortage of gene GSTM1and gene GSTT1may block the sperm production. So, geneGSTM1and gene GSTT1maybe are relevant to oligospermia.DNMT3L has been recognized as another gene that is related with spermproduction. It is important to the DNA methylati process, while during the spermproduction, the normal DNA methylati is essential to the regulative role of somerelated genes. Abnormal methylation will cause the abnormal sperm production. So,gene DNMT3L maybe is related to oligospermia.Therefore, to knowing more about the relationship between the above three genesand oligospermia, this study takes some technology, such as PCR,RELP and DNA testto do the research among those normal men who at the child-bearing age. Theresearch mainly explains the relationship between oligospermia and the gene GSTM1,deletion polymorphism of gene GSTT1, gene DNMT3L and SNP rs2070565. Someresults are as follows:1. The abasement rate of the gene GSTM1in oligospermia is higher thanthe normal men who are at the child-bearing age（68.3%vs.57.7%, χ~2=4.863, P=0.027＜0.05, OR=1.580,95%CI=1.051~2.375）。2. The abasement rate of the gene GSTT1in oligospermia is higher thanthe normal men who are at the child-bearing age（57.9%vs.46.8%, χ~2=5.025, P=0.025＜0.05, OR=1.567,95%CI=1.057~2.322）。3. The rate of the gene DNMT3L and the allelic gene A at gene rs2070565in oligospermia is obviously higher than the normal control.（20.7%vs.14.9%,χ~2=5.237, P=0.022＜0.05, OR=1.496,95%CI=1.058~2.116）。There is nomuch difference between two genotype rates.The research shows that, the abasement rate of GSTM1gene，GSTT1gene andallelic gene A at gene rs2070565are related to oligospermia. So, they may increasethe incidence of oligospermia. |
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