Baitpreparation And Immuned Neutralizing Antibody Detection Of Rabies Oral Vaccine

Rabies, also named hydrophobia, is commonly called mad dog disease, causes an acute zoonotic infectious disease by rabies virus infection in central nervous system. Rabies virus can infect all warm-blooded animals, belongs to one of infectious diseases with the highest mortality rate, almost100%of death upon infection. According to World Health Organization survey, the whole world has as many as55000people death per year by rabies, mainly in the developing countries, especially in the underdeveloped rural and mountainous areas. At present, the most effective program of rabies prevention and treatment is to inject rabies vaccine before exposure. The majority of human rabies vaccines are inactivated vaccine, while animal mainly uses attenuated vaccine with a small amount of imported inactivated vaccine. However, due to the high price and inconvenience of those injecting vaccines, prevention and elimination of rabies is extremely difficult.On the basis of the previous studies of oral vaccine in our laboratory, the biological characteristics of dogs and the experiences of oral vaccine in developed countries, this subject developed a kind of oral rabies vaccine baits using available economy resource of Guangxi Province. Upon oral immunization of dogs, the blood samples were collected and separated to obtain serum at0,14,28,42,56and70days. The immunization effect of the oral vaccine for dogs was evaluated by detecting the neutralizing antibody titer via enzyme-linked immunosorbent assay and rapid immunofluorescence assay. The results showed that oral immunization produced neutralizing antibody levels>0.5IU/ml by ELISA, emerging protective immunity, however, the neutralizing antibody levels<0.5IU/ml by RFFIT, indicating no enough protective immunity. The controversial consequences of these two methods need to be further confirmed. In short, the study proved the feasibility of oral rabies vaccine and laid a foundation for applying oral vaccine immunization of wild animals, tray dogs and domestic dogs.