The Effects Of Polar Extracts Of Uyghur Ocimum BasilicumL. On The Arachidonic Acid Metabolic Pathway In Vitro

Objective In vitro experiments to study the polar extracts of Uyghur Ocimum basilicum L. on arachidonic acid metabolic pathway, thus further its anti-inflammatory mechanism.Methods①The total flavonoids in ethyl acetate extraction and n-butanol extraction of Uyghur Ocimum Basilicum L.(OBL) enalyzed by UV Spectroscopy;To detect their removal effect on DPPH·.②Detected the cytotoxicity effect on RAW264.7cells with different polar extracts, and then determined the IC50value and dose of different parts.③The LPS-induced RAW264.7cells were interfered with OBL--ethyl acetate and OBL—butanol,The concentration of LTB4and PGE2in the supernatant was measured by ELISA and calculated the inhibition rate. Results①The total flavonoids in OBL--ethyl acetate and the OBL—butanol are21.0233μg/ml (RSDO.19%, n=5),4.7829μg/ml (RSDO.58%, n=5); For0.5mg/ml ethyl acetate extraction and n-butanol extraction, the scaveging rates of DPPH· sample were88.8%,72.2%.②The OBL--ethyl acetate and the OBL—butanol had different effects on the growth of RAW264.7cells, the IC50values were312.5a.μg/ml,350.9μg/ml respectively, the inhibition is concentration-dependent; On RAW264.7cells, followed by the maximum dose concentration of OBL--ethyl acetate and the OBL—butanol was100ug/ml in24h.③The concentration of LTB4and PGE2were inhibited by OBL-ethyl acetate and OBL--butanol extract in varying degrees, the maximal inhibition ratio on LTB4were42.78%、38.63%, the maximal inhibition ratio on PGE2were57.95%、51.0%respectively; the inhibition rates of acetiden part was stronger than the OBL--butanol (P<0.05); the inhibition rates on PGE2was higher than LTB4in (P<0.05)at the same dose. Conclusion①The flavonoids of OBL polar extracts may be a chemical components in inflammatory effects, their in vitro anti-oxidative activity to a certain extent reflect their in vitro anti-inflammatory activity.②OBL may be a dual inhibitor, which play an anti-inflammatory effects by inhibiting the activity of the enzyme cyclooxygenase-2and5-ester oxidation. OBL--ethyl acetate extract is an anti-inflammatory part of the OBL.