The Effect Of Rifapentine Solution On Proliferative Activity Of Cultured The Rabbit Adipose-derived Stem Cells

Objective: To investigate the effect of rifapentine solution on proliferativeactivity of cultured the rabbit adipose-derived stem cells in vitro conditions, andobserve whether there is a dose-effect. Methods: In this experiment we use the healthynew Zealand white rabbits (male or female),weighting2.5-2.8kg,provided by theExperimental Animal Center of Xinjiang Medical University. After anesthesia with3%sodium pentobarbital (1ml/kg) or the Sumianxin (0.3ml/kg), Ablation area hair removalshin preparation using the8%sodium sulfide, sterilization,take the scapular area ofsubcutaneous fat. Placed in petri dishes fitted with10ml PBS, brought to the laboratory. Incollagen I enzyme will cells after digestion, inoculation DMEM growth in liquid in culture.In the inverted microscope cell growth situation and shape. Stay cell proliferation tocertain degree to passaged them, then add to osteogenic agents take the thirdgenerationcells inoculation induced osteogenesis in osteoblasts induced by the first17days wereobserved the formation of calcium nodules, Alizarin red staining positive, conformed thatthe cells were rabbit adipose-derived stem cells. And then take the third generation ofadipose stem cells, respectively, three different sets of rifapentine concentration (based oneach group of the rifapentine different concentrations, divided into10μg/ml group, the the20μg/ml group, the40μg/ml group) and control group cell culture, and take the CCK-8colorimetric method to compare the proliferation and differentiation activity. Osteogenicdifferentiation ability was tested by alkaline phosphatase (ALP) staining andmineraliznodule staining. Results: ALP staining and mineralized nodule staining showedpositive. By contract to control group: Rifapentine solution concentration of10μg/ml,20μg/ml,40μg/ml there is no significant difference on cell proliferation activity(P＞0.05);Under the microscope can be observed when the rifapentine concentration of40μg/mlthere were significantly form change on adipose stem cells. Conclution: Separate from rabbit fat tissue separation, cultivate fat stem cells (ADSCs) has become the in vitroinduction osteoblast differentiation potential (OB). Rifapentine solution concentration of10μg/ml,20μg/ml there is no significant difference on cell proliferation activity; Under themicroscope can be observed when the rifapentine concentration of40μg/ml there weresignificantly form change on adipose stem cells.