Experimental And Clinical Research Of Low-dose Chemotherapy With Aidi Injection In The Treatment Of Non-small Cell Lung Cancer

Objective:Through the observation of Aidi injection in combination with Low-dose of chemotherapy treatment the clinical curative effect of lung cancer, compared with traditional Chinese medicine Aidi injection non-toxic dose small dose chemotherapy gemcitabine drug merger of lung cancer cells A549before and after the growth inhibition, and preliminary exploration the possible mechanism for Aidi injection with low-dose of chemotherapy treatment for lung function to provide the scientific connotation.Methods:1.Low dose of gemcitabine was combined with Aidi injection on lung cancer A549cell growth inhibition and apoptotic mechanismLung cancer A549cells were divided into control group, low doses of gemcitabine group (GEM30μg/ml), low doses of gemcitabine coast of the United Eddy (GEM30μg/ml+5%Aidi injection), conventional-dose gemcitabine gemcitabine group (GEM60μg/ml) the cell inhibitory rate, apoptosis rate of flow cytometry, immunocytochemistry observe the expression of Fas, bcl-2, p53protein expression, PCR measurement of cyclinDl and CDK4mRNA expression using the MTT assay.2.Low doses of chemotherapy drugs in combination Aidi injection treatment Non-small cell lung cancerA randomized, controlled clinical studies will be included in the72patients were divided into two groups, control group36cases chemotherapy with conventional-dose NP program or GP programs or the DP program. The observation group of36cases in the low-dose chemotherapy (chemotherapy regimen with the chemotherapy group, a dose of80%of the conventional dose) plus Aidi injection. After two cycles, two groups were observed term evaluation, the symptoms to improve the situation, quality of life to improve the situation, chemotherapy toxicity and other indicators.Results:1.Low dose of gemcitabine was combined with Aidi injection on lung cancer A549cell growth inhibition and apoptotic mechanismThe low dose gemcitabine joint Aidi group, the regular doses gemcitabine group all can effectively restrain A549cells to grow lung cancer, inhibition rate of11%,18%and24%respectively; Immune cell chemistry shows, group compared with normal, the treatment group Fas,p53express significantly increased, bcl-2express reduced; The comparison between treatment group, all has the small dose gemcitabine joint eddy group for comparison, small dose gemcitabine Fas expression of significantly reduced, regular doses gemcitabine express group significantly increased; Small dose gemcitabine bcl-2, p53group there were no significant difference of expression, regular doses gemcitabine bcl-2expression of significantly reduced, p53express significantly increased. With small dose gemcitabine compared group, small dose gemcitabine joint eddy treatment group cyclinD1and CDK4mRNA significantly increased expression; And regular doses gemcitabine comparison group, small dose gemcitabine joint eddy no significant differences between treatment group.2.Low doses of chemotherapy drugs in combination Aidi injection treatment Non-small cell lung cancer The trial group and control group of the total effective rate (CR+PR) were33.33%,25.00%, and disease control rates (CR+PR+SD) were83.33%,66.67%, two group total effectiveness and WenDingLv compared, and no significant differences. And in the main symptoms improved, the observation group for dry cough, bosom frowsty, dry mouth three symptoms improved significantly. Improvement of life quality (reference KPS rating), the observation group of the effective rate was77.78%to50.00%in the placebo group. In the leukopenia, oral reaction and nausea and vomiting these three aspects, two groups of comparisons are statistically significant differences. Conclusion:1.Low dose of gemcitabine was combined with Aidi injection on lung cancer A549cell growth inhibition and apoptotic mechanismAidi injection and small doses of chemotherapy gemcitabine share, can enhance the cases of cancer cell cytotoxic effects, increased sensitivity to chemotherapy, improve the drug curative effect, its mechanism may and induce the apoptosis related, main may be increased the expression of Fas, cut cyclinDl and CDK4mRNA expression.2.Low doses of chemotherapy drugs in combination Aidi injection treatment Non-small cell lung cancerLow doses of chemotherapy share Aidi injection, better than simple small dose of chemotherapy short-term curative effect, and can improve the clinical symptoms and reduce the adverse reaction of chemotherapy drugs, improve the quality of life of the patients and prognosis.