Detection Of Serum IL-17、IL-22、sFas、sFasl And T-cell Subgroup Distribution Type In Patients With Chronic Actinic Dermatitis

Objective:To detect the expression of serum IL-17、IL-22、 sFas、sFasl and T lymphocyte subgroup in peripheral blood of the patients with chronic actinic dermatitis and explore the pathogenesis of chronic actinic dermatitis. Methods:1. The enrollment of research object:24cases of chronic actinic dermatitis patients which meeting the clinical diagnostic criteria of the standard of Norris, Howk, and that of the1992Shanghai Huashan Hospital were collected and14healthy subjects were selected.2. Acquisition and preparation of blood products:a. The specimen collection of T lymphocyte subsets:5ml venous blood of chronic actinic dermatitis or healthy volunteers was adopted, anticoagulated with heparin and immediately sent for inspection. b. The specimen collection of serum IL-17、IL-22、Fas、FasL:5ml venous blood of chronic actinic dermatitis or healthy volunteers was adopted, centrifuged at3000rpm for10min after being rested at room temperature for2hours. Then lml upper serum was collected and stored at-20℃.3. The detection of blood products:a. The detection of T cell subsets: Immunofluorescence monoclonal antibody (CD4-FITC/CD8-PE/CD3-PE-Cy5) was used to fully mark anticoagulant collected and the Beckman Coulter FC500flow cytometer was used to detect respectively the levels of T lymphocyte subsets in peripheral blood of chronic actinic dermatitis or healthy volunteer according to the kit instruction. b. The detection of serum IL-17、IL-22and Fas、FasL:First, Reagents including the standard solution were prepared according to the reagent instruction. Then, A series of steps were implemented, such as adding sample, microplate washing, water bathing, microplate washing, enzyme labeling, incubation, microplate washing and dark reaction. Last the absorption value of OD at450nm was measured with a microplate reader and the levels of IL-17、IL-22、sFas、sFasl were detected. Results:1. Peripheral blood T lymphocyte subsets:CD3+CD4+of patients with chronic actinic dermatitis was significantly lower than that of the healthy group and there is a significant difference by statistical analysis (P<0.05). CD3+CD8+of patients with chronic actinic dermatitis was significantly higher than that of the healthy group and there is a significant difference by statistical analysis (P<0.05). CD3+CD4+/CD3+CD8+of patients with chronic actinic dermatitis was significantly lower than that of the healthy group and there is a significant difference by statistical analysis (P<0.05). CD3+of chronic actinic dermatitis patients and that of healthy group, no significant difference by statistical analysis (P>0.05).2. Serum IL-17、 IL-22and Fas、FasL:IL-22of patients with chronic actinic dermatitis was significantly higher than that of the healthy group and there is a significant difference by statistical analysis (P<0.05). Serum sFas of patients with chronic actinic dermatitis was significantly lower than that of the healthy group and there is a significant difference by statistical analysis (P<0.05). Serum sFasl of patients with chronic actinic dermatitis was significantly lower than that of the healthy group and there is a significant difference by statistical analysis (P<0.05). IL-17of chronic actinic dermatitis patients and that of healthy group, no significant difference by statistical analysis (P>0.05).Conclusion:There are expression imbalance of T lymphocytes and disturbance of immunological function in patients with chronic actinic dermatitis. CD8+T cells play an important role in delayed-type hypersensitivity of chronic actinic dermatitis.2. IL-22participates in the chronic inflammation and allergic reactions of chronic actinic dermatitis and promotes the development of the disease. IL-17does not play the role of anti-inflammatory or sensitization in the old chronic actinic dermatitis.3. Abnormal apoptosis proteins play a role in the development of chronic actinic dermatitis.