| Objective: Through the detection of the content of the ANGⅡ,ET-1in plasma,the C-reactive protein,Nitricoxide in serum and the expression of AT1R inaortic smooth muscle,investigate the hypotensive mechanism of Zorkin woodnourishing Yin and liver kidney hyperactivity renal hypertensive rats.Methods:Seventy-eight cases of two month old healthy male SpragueDawley（SD） rats were randomly divided into sham operation group thirteencases and sixty-five cases of the surgical group.Sham operation group thirteencases only theseparation of the left renal artery ligation,the surgical groupwere treated with ligation of leftrenal arteryrenal hypertension model,twoweeks later,through the tail cuff method to measure the rats’ bloodpressure,the successful model of the rat plus irrigation Aconite Decoction offour weeks to prepare hyperactivity card model.Model of hyperactivity cardhypertensive rats were randomly divided into model group,captoprillgroup,nourishing Yin group（Zi Yin group）,calming liver yang tonic group（referred to complement liver and kidney group）,nourishing Yin and calmingliver yang nourishing liver and kidney group（referred to as the consolidatedgroup）,were housed separately.Grouping orally administered once daily,a totalof4weeks.Sham operation group and model froup were givennormal salineorally at the asme time.24hours after the last dose, the abdominal aortic blood 8ml, to prepara serum and plasma.Using colorimetric to determin serum NOconcentration, concentrations of serum CRP measured by radioimmunoassay,concentration of plasma ET-1and ANGⅡ were measured by ELISA.Afterblood was collected, kill the rats immediately, take the thoracic aorta,usingimmunohistochemical methods to detection of thoracic aortic AT1Rexpression,HE staining under the light microscope to observe the changes inthe intima.Results:1.General observation during the experiment, shamoperation group rats coat shiny, agile.2.The change of each treatment groupstowards blood pressure of hyperactivity renal hypertensive rats.①The basis ofblood pressure The blood pressure of model group,the captopril group,ZiYin group,liver and kidney group,consolidated group compared with the shamgroup had no statistically significant（P>0.05）.②The blood pressure after2weeks of the surgery the blood pressure of rats in model group,compared withthe sham operation group,each treatment group were significantly increased（P<0.05）.③Changes in blood pressure after treatment in each group After4weeks of treatment,compared with the model group,the blood pressure of ratsin the captopril group,liver and kidney group and integrated group wassignificantly reduced（P<0.05）;Zi Yin group reduced but had no significant（P>0.05）,compared with captopril groups,the integrated group hadstatistically significant（P<0.05）,liver and kidney rats had no statisticallysignificant（P>0.05）.3.The influence of nourishing Yin and liver kidneytowards plasma ANG Ⅱ,ET-1and serum CRP,NO of hyperactivity renal hypertensive rats①compared with the sham operation group,the plasma ANGⅡ,ET-1and serum CRP of rats in model group were significantly increasedand had statistically significant （P<0.05）,serum NO of rats in model groupcompared with the sham group were significantly reduced and had statisticallysignificant（P<0.05）.②After treatment,compared with the model group,plasmaANG Ⅱ,ET-1and serum CRP of rats in captopril group,liver and kidney groupand integrated group was significantly lower（P<0.05）;serum NO wasincreased（P<0.05）.Zi Yin group had no significant（P>0.05）.③compared withcaptopril groups,integrated group had statistically significant（P<0.05）,liverand kidney group had no statistically significant（P>0.05）.4.The influence ofnourishing Yin and liver kidney towards the AT1R expression in Aortic smoothmuscle of hyperactivity renal hypertensive rats①Compared with the shamgroup,model group were significantly increased （P<0.05）.②Compared withmodel group,captopril group,liver and kidney group and integrated groupwere significantly lower（P<0.05）,Zi Yin group had no significant（P>0.05）.③Compared with the captopril group,consolidated group had statisticallysignificant（P<0.05）,liver and kidney group had no significant （P>0.05）.5.Theinfluence of nourishing Yin and liver kidney towards the thickness of themembrane of thoracic aorta of hyperactivity renal hypertensive rats①Compared with the sham group,the thickness of the membrane of thoracicaorta of rats in model group were significantly increased（P<0.05）.②Compared with model group, captopril group,liver and kidney group were significantly lower（P<0.05）,Zi Yin group had no significant（P>0.05）.③Compared with the captopril group,consolidated group had statistically significant（P<0.05）,the liver and kidney group had no significant（P>0.05）. Conclusion:1.Through incomplete ligation of the left renal artery,leading to the renal artery stenosis,renal hypertensive rats were successfully prepared.Through irrigation aconite soup to the RHR successfully prepared hyperactivity rat model.2.Nourishing Yin and liver kidney has obviously antihypertensive effect on hyperactivity renal RHR,has the effection of inhibition the remodeling of vascular wall.3.Mechanism with the following factors:①The reduced vasoconstrictor the ET-1formation,increase the vasodilator NO production,lower blood pressure.②Reduce the formation of serum CRP levels,reduce the endothelial damage due vessel wall thickening,reduced compliance.③Reducing of ANG Ⅱ formation,reduce AT1R expression in vascular smooth muscle,lower blood pressure,and inhibit vascular wall thickening and reconstruction. |
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