The Effect Of The Fasudil Hydrochloride Injection On The Scar Models Of The Rabbits’ Ears

Objective:The experiment through comparing anddisgussing the fasudil hydrochloride injection(FHI),the triamcinoloneacetonaide acetate injection(TAAI) and the normal saline(NS),which areused to inject into the hypertrophic scars on the rabbits’ ears.Observingfrom the general and histopathological sight and statisical analyzing thehypertrophic index(HI), the fibroblast index(FBI) and the distribution ofthe collagen fibers(CF) to do the research on the inhibitory effect of theFHI on the hypertrophic scars tissue of the rabbits’ ears.Methods:12healthy standardization Janpanese rabbits,a total of24ears,half male andhalf female.All of them weight about2.0-2.5kg and are at the age of8to12months. Give the anesthesia by Ketamine1mg/kg intramuscularinjection after the rabbits random grouping into three groups,4rabbits foreach group.Creat the hyperplastic scar model on the rabbits’ ears,eachrabbit ears make4models like this,96models in all.In day28of theexperiment,randomly chose1proliferative tissue for pathological stainingto confirm the hyperplastic scar has been made successfully.Eachproliferative plaque is injected separately within the FHI,TAAI and NS bydose of30μl each proliferative plaque in day28,35,42during theexperiment.Observeing and recording while in the process of drug scarshape, size, quality of a material.In day49of the experiment kill the rabbits,take hyperplastic scar tissue samples to HE staining and Massonstaining.Observing the formationg and distribution of the fibroblasts andcollage fibers under the medical microscope.Caculate the number of thefibroblasts and the hypertrophic index through the medicalmicroscope.The experimental datas and pictures will be statisticanalysised by PASW Statistics18(SPSS18.0) statistical software.Results:1, General performance: NS group still has the obvious skincontracture and the scars are more high than the others.The scars are darkqualitative,hard, subcutaneous of obvious nodularity and can, unevensurface. FH and TAA groups’ scar tissues slightly higher than the leatherface, did not see the obvious contracture,.The color of the scars becomesshallow, close to light, quality of a material is become soft, subcutaneousnodules small, between the two groups scar atrophy no obviousdifference degree.2,HE staining viewed by microscope:there is aincreased number of the capillaries and fibroblasts more than the FH andTAA groups. A loose orderly arrangement of collagen fibers, unlike thecollagen fibers arregment in the NS group.The nucleus has not seen theobvious increase, cells are arranged a close and orderly.3, Massonstaining viewed by the microscope:the collagen fiber in the NS group isdeep blue stained, the cell distribution is disordered.The blue staining inNS group is darker than in the FH and TAA groups.Between FH groupand TAA group,the two groups’ collagen fiber,the morphology, distribution, staining have not been seen a obvious difference.4, Thehyperplasia index and the number of the fibroblasts count in NS group arehigher than which in the FH and TAA group, two difference between thetwo groups have a statistically significant (P <0.05).Compared with FHand TAA two groups, the scar hyperplasia index has no significantdifference (P>0.05) between FH group and TAA group.The FHcomposition fiber cell count is higher than TAA group, two differencebetween the two groups have statistically significant (P <0.05).We takethe point that the TAAI have inhibitory effect on fibroblasts moreremarkable that FHI.Conclusion: the fasudil hydrochloride injection(FHI)and the triamcinolone acetonaide acetate injection(TAAI) have asignificant inhibitory effect on the hypertrophic scar models of therabbits’ ears.They can make the scar tissue become soft and flat,anddecrease the hypertrophic index.The TAAI has a more significantinhibitory effect on the fibroblasts more than the FHI.As a new type ofthe calcium antagonist,the fasudil hydrochloride injection(FHI) can beused for clinical treatment of hyperplastic scar as another securityeffective drug treatment choice for treating the hyperplastic scars.