Experimenta1Study Of Gabexate Mesilate Protects Against Sma11Intestina1Ischemia-reperfus1on Injury In Rats

Objective: a broad-spectrum protease inhibitor gabexate mesilate(GM) as a new type of enzyme inhibitors, can inhibit a variety of proteases,thevasodilatory peptide, trypsin, fiber protease, esterase C1, and phospholipaseA2was inhibited by inhibiting release of inflammatory mediators. Small intestinalmucosa through research gabexate intestinal ischemia reperfusion injury in thedegree of influence on intestinal ischemia-reperfusion (ischemia／ruperfusion,IR), small intestinal TNF-a,IL-6, malondialdehyde (MDA)expression. Method:Healthy adult SD rats were42, the weight between280g-320g, were male,were randomly divided into control group, ischemia-reperfusion (IR) groupand the gabexate intervention group. Depilatories off the hair of abdominalarea, and routine disinfection shop towels to take on the abdominal midlineincision of about2-3cm into the abdominal cavity after the rapid separation ofthe superior mesenteric artery (SMA). Control group separation of SMA Afterthe abdomen was closed again after45minutes to open the abdomen wasclosed, not the other treatments.IR group, non-invasive arterial clip clampingthe SMA,45min afterremoval of the arterial folder reperfusion. Gabexategroup by clamping the SMA the45min underwent reperfusion, in the top40min reperfusion by the tail vein into the GM with the 【10mg／(kg·h)】 of content.2hours of reperfusion to collect specimens respectively. Smallintestinal tissue pathology testing HE dyed, ELISA test rats serum workTNF-a, IL-6content., In living circumstances of blind from the back cut offsmall intestine3cm, determination of the basis of MDA levels. Results:(1)The intestinal mucosa pathological changes: healthy rats small villi feelcomplete, no significant bleeding and fall off without obvious inflammatorycells infiltration and blood capillary dilate. IR rats, small villi feel with thenatural layer on top of epithelial separation, the inherent layer bleeding, a largenumber of inflammatory cells infiltration, mucous membrane fluffy necrosisfall off. gabexate mesilate group of bowel mucosa damage nap group is lighter,small intestinal mucosa capillary mild expansion, passive congestion, part ofthe increase in the gap with epithelial cortex layer with inherent moderateseparation, inflammatory cells infiltrating less. The control group, IR groupand the gabexate group of the pathology of Chiu score’s score, respectively:0.92±0.55,4.21±0.51,2.42±0.54, each group has tested statistically（P<0.05）.(2) Cell factors change: the control group, IR group and the gabexate groupischemia-reperfusion two hours after rats in the serum cell factors TNF-arespectively is:25.60±2.58pg/ml,73.49±24.90pg/ml,42.23±13.48pg/ml,each group has tested statistically（P<0.05）. Rats in the serum cell factors IL-6respectively is:16.31±3.13pg/ml70.23±23.70pg/ml34.69±13.56pg/ml, eachgroup has tested statistically（P<0.05）.(3)The control group, IR group and thegabexate group ischemia-reperfusion two hours after intestinal tissue of the rats MDA content are:3.20±0.73nmol/mgprot,10.47±1.30nmol/mgprot,4.96±0.82nmol/mgprot,each group statistically （P<0.05）.Conclusion:(l)Gabexate effective to reduce the small intestinal pathology in rat intestinalischemia-reperfusion-induced injury severity: the small intestinal villi degreeof necrosis, reduce the fluff off, reducing the infiltration of neutrophils, reducemucosal bleeding.(2)Gabexate inhibited ischemia-reperfusion, oxidation andreduce the MDA content of rat intestinal tissue.(3)Gabexate reduces intestinalischemia reperfusion injury of proinflammatory cytokines TNF-a, IL-6release.(4) Gabexate may reduce the intestinal inflammation caused by injury, has aprotective effect on intestinal ischemia-reperfusion injury.