The Effect Of Batroxobin And Butylphthalide On The Cerebral Ischemia Injury In Rats

Objective:Cerebrovascular disease is a neurological disease that cerebral vascular lesions caused by various factors. It is clinical common, frequently-occurring disease, the mortality and morbidity are high, which is the third leading cause of death. Therefore, research on the prevention and treatment of ischemic cerebrovascular disease is a major issue of concern for human health. The clinical treatment of the key is to save the ischemic region (penumbra) of dying neurons and promote damage nerve function recovery. Explore the cerebral protective effect of Batroxobin and Butylphthalide on acute cerebral infarction and its possible mechanism; Investigate the effect of the Batroxobin United Butylphthalide on the treatment of acute cerebral infarction.Methods:100adult Wistar rats are randomly diveded into five groups:common group(group A), cerebral ischemia group(group B), batroxobin group(group C), butylphthalide group(group D) and combination group(group E). The rats subjected to middle cerebral artery occlusion with nylon suture occlusion, inject intraperitoneally with normal saline about4ml/kg0.5hours after infarction(group B), inject intraperitoneally with tobishi batroxobin about8BU/kg0.2hours after infarction(group C and E), inject intraperitoneally with edaravone about8mg/kg0.5hours and6hours after infarction(group D and E). Remove the brain after infarction24hours later and dyeing in TTC buffer. Normal tissue dyed pink,infarction dyed white. Photograph these specimen,and calculate the infarct size using LUZEX-F image analysis software.Result:1.Process the rats with Longa’neurobehavioral score2hours after infarction, there are significant differences in batroxobin group, butylphthalide group and cerebral ischemia group, in combination group and cerebral ischemia group(P<0.05); There is a significant difference in batroxobin group and butylphthalide group(P<0.05); The combination group and batroxobin group are also significantly different.2.D-dimer in cerebral ischemia group is significantly higher than the common group(P<0.05); The butylphthalide group is significantly lower than the cerebral ischemia group(P<0.05); The batroxobin group is significantly lower than the butylphthalide group(P<0.05); The combination group is significantly lower than the batroxobin group(P<0.05); The common group is significantly lower than the combination group(P<0.05).3. D-dimer in cerebral ischemia group is significantly higher than the common group(P<0.05); The butylphthalide group is significantly lower than the cerebral ischemia group(P<0.05); The batroxobin group is significantly lower than the butylphthalide group(P<0.05); The combination group is significantly lower than the batroxobin group(P<0.05); The common group is significantly lower than the combination group(P<0.05).Conclusion:By reducing the D-dimer and ultra-sensitivity C-reactive protein levels, Tobishi batroxobin and butylphthalide can reduce the infarct size of acute cerebral infarction. Tobishi batroxobin and butylphthalide have a cerebral protective effect, and combination therapy better.