Protection Of Maslinic Acid Against Oxygen And Glucose Deprivation In Rat Cortical Neurons

Objective:To explore the protection of maslinic acid against oxygen and glucose deprivation(OGD) in rat primary cortical neuron.Methods:Primary cortical neuron dissociated from Sprague-Dawley rats15-17embryonic days were identified by Tuj1on the5th day after culture and observed on the6th day after culture. Replaced the culture media with DMEM solution that contained high-glucose or didn’t contain glucose.Placed the group with glucose in a humidified incubator containing normoxia.That group withotut glucose was placed in a modified anaerobic chamber which an anaerobic gas mixture (5%CO2+95%N2),Treated0、3、6、9hours. Detected the release of LDH and MTT assay after OGD for cell injury and cell viability to find the appropriate treatment time. In the appropriate treatment time point of the OGD model, Maslinic Acid in0、0.1、1、10μM Pre-treat the OGD model, Detected the release of LDH and MTT assay after OGD for cell injury and cell viability to find the protection of maslinic acid against OGD in rats primary cortical neurons, apoptosis related protein-Bax was detected by Western Blot analysis。Results:The establishment of OGD model is successful and the appropriate treatment time of OGD model is6h,Treatment with Maslinic Acid0.1、1、10μM in the OGD group reduced the amount of LDH release,MTT assay was also significantly increased in1、10μM, comparing with the OGD+OμM Maslinic Acid.Western Blot:The reducing of the expression of Bax with the increasing of the concentration of the maslinic acid.Conclusion:Treatment with maslinic acid has protective effect to cell injury induced by OGD and it may be correlated to decreasing of the expression of Bax.