Chemical Constituents Of Three Endolichenic Fungi

Microorganism isolated form the healthy internal issues of their hosts mainly includes fungi, bacteria, and actinomycetes, typically causing no apparent symptoms of disease. Endofungi have been investigated deeply and considered as important sources of sustainable, bioactive, and chemically novel secondary metabolites. Endolichenic fungi, like endophytic fungi residing inside healthy plant tissues, live within the asymptomatic lichen thallus. Endolichenic fungi are commonly ignored and should be largely untapped resources. However, endolichenic fungi are attracting more and more attention since the first report of their metabolites in2007.In this paper, three endolichenic fungal strains were subjected to be fermented for chemical investigation. A total of37secondary metabolites, mainly including13perylenequinones,3terphenylquinones,2fatty acids, and8steroids, were isolated by column chromatography (CC) on silica gel and Sephadex LH-20, as well as by HPLC.12of them were new compounds, including7perylenequinones,3terphenylquinones,1fatty acid, and1α,β-unsaturated ketone. The structures of these new compounds were elucidated by spectroscopic data including MS, NMR, UV, and IR, as well as TDDFT CD calculations. Some of isolated compounds were screened for their cytotoxic and antifungal activities. Perylenequinones exhibited strong cytotoxicity against PC3, DU145, and LNCaP cell lines with accumulating in the lysosomes.