Effects Of Mild Hypothermia On Expression Of Nuclear Transcription Factor-kappab (NF-κB) In Rat Cerebral Microvascular Endothelial Cells Injured By Hydrogen Peroxide

Background:With the development of human society and its increasing longevity,the incidence of intracerebral haemorrhage (ICH) increased year by year and is getting an enormous socioeconomic problem for the future. As the most devastating subtype of stroke,ICH accounts for15%～20%of all deaths from stroke.Although great progress we have made in treatment of stroke during the past decades,there is still lack effective treatment strategy for ICH except blood pressure control,management of edema and prevention of complications. So,it has become a hot spot to study the underlying brain injury induced by ICH. At present, mild hypothermia is thought to be the most promising neuroprotective treatment for stroke as it can afftect multiple aspects of intracerebral hemorrhage injury. There is growing evidence showing that mild hypothermia could reduced edema, blood-brain barrier disruption, and inflammation, while inflammation play an crucial role in the secondary brain injury after ICH.In this article we established cell model of inflammatory response after cerebral hemorrhage to investigate the possible protective mechanisms of mild hypothermia.Objective:To investigate the influence of mild hypothermia on expression of nuclear factor-kappaB (NF-κB) in cerebral microvascula endothelial cells injured by hydrogen peroxide Methods:The cells of rat cerebral microvascular endothelial were separated and nurtured, and cell model of inflammatory response after cerebral hemorrhage was established by the method of hydrogen peroxide injury. Cells were divided into experiment group (ICH)and control group(C). Each group was divided into normothermia group (N) and mild hypothermia group (H). The expression of NF-κB was detected by western blotting after hydrogen peroxide intervention.Results:The expression of NF-κB was increased obviously in experiment group than that in control group (p<0.01, p<0.01). While the level of NF-κB was higher in nomothermia group than that in mild hypothermia group(p<0.01).Conclusion Mild hypothermia may protect perihematoma tissues through relieve the inflammation by inhibit NF-κB overexpression.