The Relationship Between The Expression Of PIGF And MVD In Human Intrahepatic Cholangiocarcinoma

Background Intrahepatic cholangiocarcinoma (ICC) is the secondmost common primary liver cancer,and its incidence and mortality havebeen increasing globally. Most patients with ICC usually have advanceddisease at the time of diagnosis because of the aggressive nature of thistumor,and the5-year survival rate is only22-36%even after curativesurgical resection.A number of studies have revealed various pathologicaland biological prognostic factors in ICC,but the precise reason for itsbiological aggressiveness remains unclear.Objective To study the relationship between the PlGF expression andMVD in human intrahepatic cholangiocarcinoma(ICC).Methods The expression of PlGF and CD34were examined byimmunohistochemistry in50human intrahepatic cholangiocarcinomatissues and8normal liver tissues.Then the microvessel density(MVD) ofhuman intrahepatic cholangiocarcinoma tissues and the portal area ofnormal liver tissues were determined.Results The (+) and (++) expression rate of PlGF were54%and28% in intrahepatic cholangiocarcinoma tissues,25%and0%in biliaryepithelium of normal liver tissues， The MVD of intrahepaticcholangiocarcinoma tissues and the MVD of the portal area of normal livertissues were52.88±21.40and14.86±3.38.The (+) and (++) expression rateof PlGF in intrahepatic cholangiocarcinoma tissues were significantlyhigher than that in normal liver tissues,the MVD of intrahepaticcholangiocarcinoma tissues were significantly higher than that of the portalarea of normal liver tissues,(P<0.05).The MVD of PlGF(++) group、PlGF(+) group and PlGF (-) were65.36±22.87、51.80±18.12and36.68±17.80,the MVD of PlGF(++) group>PlGF(+) group>PlGF(-)group,the differences were significant(P<0.05).Conclusion PlGF is relate to ICC,The increased PlGF expressionmay be one of the factors that promote angiogenesis in human ICCs.