Effect Of Drynariafortunei Naringin Combined With Panax Notoginseng Saponins On The Differentiation And Ultra-Structure Of Human Periodontalligament Cells

Objective To eveluate the effects of Drynaria fortunei naringincombined with Panax notoginseng saponins on the differentiation andultra-stucture of human periodontal ligament cells.Methods (1)Through tissue-culture method,primarily culture andidentify the human periodontal ligament cells by immu-unohistichemicalmethod.(2)MTT method was detected to observe the proliferation ofhPDLCs with the effects of different concentrations of Drynaria fortuneinaringin,Panax notoginseng saponins and the combination.(3)Enzymekinetics method was adapted to investigate the effect of Drynaria fortuneinaringin,Panax notoginseng saponins,combination of Drynaria fortuneinaringin and Panax notoginseng saponins on the alkaline phosphataseactivity of hPDLCs.(4)Western blot was used to detect the expression ofalkaline phosphatase.(5)Transmission electron microscope was used tostudy the ultra-structure of hPDLCs with the effects of Drynaria fortuneinaringin,Panax notoginseng saponins,combination of Drynaria fortuneinaringin and Panax notoginseng saponins. Results (1)hPDLCs was cultured by tissue-culture method,examinedby immunohistochemistry:vimentin positive and anti-CK negative.(2)Dryn-aria fortunei naringin,Panax notoginseng saponins,combination of Drynariafortunei naringin and Panax notoginseng saponins could promtote theproliferation,the optimal value of Drynaria fortunei naringin was1mg/L,the optimal value of Panax notoginseng saponins was1mg/L, thecombination group was better than the others.(3) Drynaria fortuneinaringin,Panax notoginseng saponins,combination of Drynaria fortuneinaringin and Panax notoginseng saponins could increase the alkalinephosphatase activity,the combined was the best.(4)Drynaria fortuneinaringin,Panax notoginseng saponins,combination of Drynaria fortuneinaringin and Panax notoginseng saponins could promtote expression ofalkaline phosphatase,the best is the combined.(5)More rough endoplasmicreticulum and ribosome in the experimental group than in the control,thecombined was the best.Conclusion Drynaria fortunei naringin and Panax notodinsengsaponins can be combined for therapy and play synergistic effect.